Investigation of OXA Type Beta-Lactamases and PFGE Patterns in Acinetobacter baumannii Strains Resistant to Carbapenems

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dc.contributor.authorKeyik, Serafettin
dc.contributor.authorArslan, Ugur
dc.contributor.authorDagi, Hatice Turk
dc.contributor.authorSeyhan, Tuba
dc.contributor.authorFindik, Duygu
dc.date.accessioned2020-03-26T18:51:15Z
dc.date.available2020-03-26T18:51:15Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAcinetobacter baumannii is an important opportunistic and multidrug-resistant pathogen leading to nosocomial infections. Over the last 10 years, a significant and threatening increase in resistance to carbapenems, mainly due to the dissemination of class D beta-lactamases, has been reported in A.baumannii worldwide. The most common types of beta-lactamases causing carbapenem resistance in A.baumannii are the OXA-23, OXA-24, OXA-40, OXA-58 and OXA-143 type serine beta-lactamases. The aim of this study was to investigate the presence of OXA type beta-lactamases in carbapenem-resistant A.baumannii strains and the clonal relationship between the strains. A total of 105 non-duplicate carbapenem-resistant A.baumannii strains isolated from various clinical samples (68 blood, 18 bronchoalveolar lavage, 13 drainage, 3 urine, 2 cerebrospinal fluid and 1 catheter samples) in the Microbiology Laboratories of Selcuk University, Meram (2009-2012) and Selcuklu (2007-2008) Medical School Hospitals, were included in the study. The isolates were identified by conventional methods and Phoenix 100 BD (BD Diagnostic, USA) and Vitek II (bioMerieux, France) automated systems. Carbapenem susceptibility test was performed by Kirby-Bauer disk diffusion method according to the CLSI standards. bla(OXA 23-like), bla(OXA 24-like), bla(OXA 58-like) and bla(OXA 51-like) genes were amplified by multiplex PCR assay and clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE) using Apal enzyme. The bla(OXA 51-like) gene was determined in all carbapenem-resistant A.baumannii isolates, while the bla(OXA 23-like) and bla(OXA 58-like) genes were detected in 46.6% and 53.3% of isolates, respectively. However biG(OXA) (24-like) gene was not demonstrated in any isolates. bla(OXA 23-like) gene was determined in both Meram and Selcuklu Medical School hospitals, but bla(OXA 58-like) gene was detected only in Meram Medical School hospital. PFGE analysis of the isolates revealed 32 different groups in bla(OXA 23-like) producing A.baumannii strains and 23 different groups determined in bla(OXA 58-like) producing strains. No common epidemic isolates were detected in the two hospitals, however it was noted that some clones produced small outbreaks in Meram MS hospital. In this study it was shown that bla(OXA 23-like) and bla(OXA 58-like) genes together with bla(OXA 51-like) gene had significant roles in the carbapenem-resistance of A.baumanniistrains. Carbapenem-resistant A.baumannii strains producing bla(OXA 23-like) and bla(OXA 58-like) enzymes showed the epidemic potential of this nosocomial pathogen and the requirement of molecular typing methods to identify the epidemiologic relationship of the isolates.en_US
dc.identifier.endpage565en_US
dc.identifier.issn0374-9096en_US
dc.identifier.issue4en_US
dc.identifier.pmid25492651en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage556en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30948
dc.identifier.volume48en_US
dc.identifier.wosWOS:000345811400004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherANKARA MICROBIOLOGY SOCen_US
dc.relation.ispartofMIKROBIYOLOJI BULTENIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectcarbapenem resistanceen_US
dc.subjectOXA type beta-lactamasesen_US
dc.subjectmolecular epidemiologyen_US
dc.subjectPFGEen_US
dc.titleInvestigation of OXA Type Beta-Lactamases and PFGE Patterns in Acinetobacter baumannii Strains Resistant to Carbapenemsen_US
dc.typeArticleen_US

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