Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts

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dc.contributor.authorÖztürk, Fırat
dc.contributor.authorMalkoç, Sıddık
dc.contributor.authorErsöz, Mustafa
dc.contributor.authorHakkı, Sema S.
dc.contributor.authorBozkurt, Buket S.
dc.date.accessioned2020-03-26T18:15:54Z
dc.date.available2020-03-26T18:15:54Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction: The aim of this study was to evaluate the cytotoxicity of 3 orthodontic acrylic materials and 2 manipulation methods. Methods: The orthodontic acrylic materials Orthocryl EQ (Dentaurum, Ispringen, Germany), Orthoplast (Vertex Dental, Zeist, The Netherlands), and O-80 (Imicryl, Konya, Turkey) were prepared with 2 polymerization methods (doughing and spray on). Totally, 60 cylinders (5 x 2 mm), fabricated by using a different acrylic and method, were divided into 6 groups. Gingival fibroblasts were isolated from gingival connective tissue of systemically healthy subjects. Materials were incubated in Dulbecco's modified eagle's medium culture medium (Biological Industries, Beit Haemek, Israel) for 72 hours according to ISO 10993-5 standards (surface area to volume ratio of the specimen to cell-culture medium: 3 cm(2)/mL). Gingival fibroblasts were maintained with Dulbecco's modified eagle medium containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Mannheim, Germany, and ACEA Biosciences, San Diego, Calif) was used to evaluate cell survival. After seeding 200 mu L of the cell suspensions into the wells (20,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with bioactive components released by the acrylic materials (1/1 and 1/2 dilutions) and monitored every 15 minutes for 121 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance (ANOVA) and Tukey-Kramer multiple comparisons tests. Results: There was no significant difference between the cell indexes of the control and study groups for the 1/1 and 1/2 dilutions at 21 and 32 hours. When evaluated at 68 hours, all 1/2 dilutions of acrylic materials showed statistically insignificant differences (P >0.05) except for Orthoplast (P <0.05). But all acrylic materials were different from the control group in the 1/1 dilutions (P <0.001). At 121 hours, all test groups were significantly different from the untreated control group (P <0.001). Conclusions: The results indicate that the long cycle increased the cytotoxicity of the tested materials, and there was no significant difference between the spray-on and doughing methods on cytotoxicity. (Am J Orthod Dentofacial Orthop 2011; 140:e243-e249)en_US
dc.identifier.doi10.1016/j.ajodo.2011.05.019en_US
dc.identifier.endpageE249en_US
dc.identifier.issn0889-5406en_US
dc.identifier.issn1097-6752en_US
dc.identifier.issue5en_US
dc.identifier.pmid22051502en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpageE243en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ajodo.2011.05.019
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26773
dc.identifier.volume140en_US
dc.identifier.wosWOS:000296745100005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMOSBY-ELSEVIERen_US
dc.relation.ispartofAMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleReal-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblastsen_US
dc.typeArticleen_US

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