Transcriptomic expression levels of the VHL, TIMP-3, and RASSF1A genes in renal tumors

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dc.contributor.authorÜre, I.
dc.contributor.authorKonaç, Ece
dc.contributor.authorAlp, E.
dc.contributor.authorÖnen, Hacer İlke
dc.contributor.authorBatur, A. F.
dc.contributor.authorGönül, I. I.
dc.contributor.authorMenevşe, S.
dc.contributor.authorSözen, S.
dc.date.accessioned2020-03-26T20:19:38Z
dc.date.available2020-03-26T20:19:38Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractOBJECTIVE: In this study, we aimed to investigate the relation between the mRNA expression levels of VHL, TIMP-3 and RASSF1A genes, and the histopathological and clinical characteristics of patients with renal tumors. PATIENTS AND METHODS: Radical nephrectomy specimens of cases presented without neoadjuvant treatment were confirmed to be cancerous, non-cancerous, benign, and healthy after removal from separate localizations. A total of 69 patients with kidney tumors (138 tissue samples) were included in the study group. RNA isolation, reverse transcriptase PCR (RT-PCR), and quantitative real time PCR (qPCR) were performed, and the GAPDH gene was used to normalize mRNA levels. RESULTS: In the RCC cancerous tissue. TIMP-3 levels increased 1.3 times and RASSF1A levels increased 1.4 times compared to the corresponding levels in non-cancerous tissues, and there was no statistically significant difference in these values. On the other hand, VHL gene expression levels in cancerous tissue were 2.8 times higher than in matched adjacent non-cancerous tissues (p < 0.05). In the case of oncocytomas, TIMP-3 levels were found to be 3.2 times higher, RASSF1A levels 3.8 times higher, and VHL levels 2.2 times lower than the corresponding levels in healthy tissues (p < 0.05). CONCLUSIONS: The roles of VHL, TIMP-3, and RASSF1A mRNA expression in contributing to the development of renal tumors could not be clearly established. Further studies are therefore required to elucidate the mechanisms underlying renal tumors.en_US
dc.description.sponsorshipGazi University Research FundGazi University [01/2010-25]en_US
dc.description.sponsorshipThis study was conducted with financial support from the Gazi University Research Fund with the project code number 01/2010-25.en_US
dc.identifier.citationUre. I., Konac, E., Alp, E., Onen, H. I., Batur, A. F., Gonul, I.I., Menevse, S., Sozen, S. (2019). Transcriptomic Expression Levels of the VHL, TIMP-3, and RASSF1A Genes in Renal Tumors. European Review for Medical and Pharmacological Sciences, 23(21), 9313-9320.
dc.identifier.doi10.26355/eurrev_201911_19424en_US
dc.identifier.endpage9320en_US
dc.identifier.issn1128-3602en_US
dc.identifier.issue21en_US
dc.identifier.pmid31773698en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage9313en_US
dc.identifier.urihttps://dx.doi.org/10.26355/eurrev_201911_19424
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38350
dc.identifier.volume23en_US
dc.identifier.wosWOS:000499705600020en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBatur, A. F.
dc.language.isoenen_US
dc.publisherVERDUCI PUBLISHERen_US
dc.relation.ispartofEUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRenal cell carcinomaen_US
dc.subjectqPCRen_US
dc.subjectmRNA expressionen_US
dc.titleTranscriptomic expression levels of the VHL, TIMP-3, and RASSF1A genes in renal tumorsen_US
dc.typeArticleen_US

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