Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters

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dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorKeskin, Nazan
dc.contributor.authorTaspinar, Mehmet
dc.contributor.authorCoyan, Kenan
dc.contributor.authorBaspinar, Nuri
dc.contributor.authorCenariu, Mihai C.
dc.contributor.authorBilgili, Ali
dc.date.accessioned2020-03-26T18:43:06Z
dc.date.available2020-03-26T18:43:06Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10 mM raffinose, 5 mM hypotaurine, 5 mM raffinose +2.5 mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 degrees C for 25 s in a water bath for evaluation. The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 +/- 12.2%, 46.3 +/- 13.6%) compared to controls (38.8 +/- 13.8%, 30.5 +/- 11.7%, P < 0.05). For the CASA progressive motility, 5 mM raffinose (20.12 +/- 8.82%) had increasing effect in comparison to control (10 +/- 7.94%, P <0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 +/- 4.68%, 40.8 +/- 4.7%), high sperm mitochondrial activity (29.5 +/- 5.4%, 27.3 +/- 4.9%) and acrosome integrity (50.8 +/- 8.1, 50.7 +/- 4.4) percentages, compared to control groups (31.5 +/- 3.5%, 9.5 +/- 8.2%, 42.8 +/- 7.3%, P < 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 +/- 1.2% and 2.1 +/- 0.9%) than that of control (4.9 +/- 2.5%) (P < 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipSelcuk University, Directorate of Scientific Research Projects (BAP)Selcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108 O 522]en_US
dc.description.sponsorshipThis study was financed under the project supported by Selcuk University, Directorate of Scientific Research Projects (BAP) and by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 108 O 522).en_US
dc.identifier.doi10.1016/j.cryobiol.2013.04.007en_US
dc.identifier.endpage39en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue1en_US
dc.identifier.pmid23644017en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage34en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2013.04.007
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29770
dc.identifier.volume67en_US
dc.identifier.wosWOS:000321601600006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRam spermen_US
dc.subjectAdditivesen_US
dc.subjectMotilityen_US
dc.subjectFlourescent staining parametersen_US
dc.subjectComet testen_US
dc.subjectTUNEL-positive cellsen_US
dc.titleRaffinose and hypotaurine improve the post-thawed Merino ram sperm parametersen_US
dc.typeArticleen_US

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