Amperometric xanthine biosensors based on electrodeposition of platinum on polyvinylferrocenium coated Pt electrode
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Novel xanthine biosensors were successfully fabricated by immobilizing xanthine oxidase on polyvinylferrocenium perchlorate matrix (PVF+ClO4-) and platinum electrodeposited polyvinylferrocenium perchlorate matrix. PVF+ClO4- film was coated on Pt electrode at +0.7 V vs. Ag/AgCl by electrooxidation of polyvinylferrocene (PVF). Platinum nanoparticles were deposited on PVF+ClO4- electrode by electrochemical deposition in 2.0 mM H2PtCl6 solution at -0.2 V. Xanthine oxidase was incorporated into the polymer matrix via ion exchange process by immersing modified Pt electrodes in the enzyme solution. The amperometric responses of the biosensors were measured via monitoring oxidation current of hydrogen peroxide at +0.5 V. Under the optimal conditions, the linear ranges of xanthine detection were determined as 1.73 x 10(-3)-1.74 mM for PVF+XO- and 0.43 x 10(-3)-2.84 mM for PVF+XO-/Pt. The detection limits of xanthine were 5.20 x 10(-4) mM for PVF+XO- and 1.30 x 10(-4) mM for PVF+XO-/Pt. Moreover, the effects of applied potential, electrodeposition potential, H2PtCl6 concentration, amount of electrodeposited Pt nanoparticles, thickness of polymeric film, temperature, immobilization time, xanthine and xanthine oxidase concentrations on the response currents of the biosensors were investigated in detail. The effects of interferents, the operational and storage stabilities of biosensors and the applicabilities to drug samples of the biosensors analysis were also evaluated. (C) 2011 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Xanthine detection, Xanthine oxidase, Platinum electrodeposition, Amperometric biosensor, Polyvinylferrocenium
Kaynak
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
72
Sayı
03.04.2020
