Amperometric xanthine biosensors based on electrodeposition of platinum on polyvinylferrocenium coated Pt electrode

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dc.contributor.authorBas, Salih Zeld
dc.contributor.authorGulce, Handan
dc.contributor.authorYildiz, Salih
dc.date.accessioned2020-03-26T18:13:43Z
dc.date.available2020-03-26T18:13:43Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractNovel xanthine biosensors were successfully fabricated by immobilizing xanthine oxidase on polyvinylferrocenium perchlorate matrix (PVF+ClO4-) and platinum electrodeposited polyvinylferrocenium perchlorate matrix. PVF+ClO4- film was coated on Pt electrode at +0.7 V vs. Ag/AgCl by electrooxidation of polyvinylferrocene (PVF). Platinum nanoparticles were deposited on PVF+ClO4- electrode by electrochemical deposition in 2.0 mM H2PtCl6 solution at -0.2 V. Xanthine oxidase was incorporated into the polymer matrix via ion exchange process by immersing modified Pt electrodes in the enzyme solution. The amperometric responses of the biosensors were measured via monitoring oxidation current of hydrogen peroxide at +0.5 V. Under the optimal conditions, the linear ranges of xanthine detection were determined as 1.73 x 10(-3)-1.74 mM for PVF+XO- and 0.43 x 10(-3)-2.84 mM for PVF+XO-/Pt. The detection limits of xanthine were 5.20 x 10(-4) mM for PVF+XO- and 1.30 x 10(-4) mM for PVF+XO-/Pt. Moreover, the effects of applied potential, electrodeposition potential, H2PtCl6 concentration, amount of electrodeposited Pt nanoparticles, thickness of polymeric film, temperature, immobilization time, xanthine and xanthine oxidase concentrations on the response currents of the biosensors were investigated in detail. The effects of interferents, the operational and storage stabilities of biosensors and the applicabilities to drug samples of the biosensors analysis were also evaluated. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSelcuk University Research Foundation (BAP)Selcuk University [08101018]en_US
dc.description.sponsorshipThe authors are grateful for the financial support provided by the Selcuk University Research Foundation (BAP) under Project 08101018.en_US
dc.identifier.doi10.1016/j.molcatb.2011.06.017en_US
dc.identifier.endpage288en_US
dc.identifier.issn1381-1177en_US
dc.identifier.issn1873-3158en_US
dc.identifier.issue03.04.2020en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage282en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.molcatb.2011.06.017
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26100
dc.identifier.volume72en_US
dc.identifier.wosWOS:000295299800030en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATICen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectXanthine detectionen_US
dc.subjectXanthine oxidaseen_US
dc.subjectPlatinum electrodepositionen_US
dc.subjectAmperometric biosensoren_US
dc.subjectPolyvinylferroceniumen_US
dc.titleAmperometric xanthine biosensors based on electrodeposition of platinum on polyvinylferrocenium coated Pt electrodeen_US
dc.typeArticleen_US

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