Farklı dentin bonding sistemlerin antibakteriyel etkilerinin karşılaştırılması
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Tarih
2005
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada üç farklı dentin bonding sisteminin [ABF Bond (Kuraray Japonya), PQ1 (Ultradent, ABD) ve Stae (SDI, Avustralya)] antibakteriyel etkisi disk difüzyon metodu ve mikro sulandırma metodu ile değerlendirildi. Gereç ve Yöntem: Çalışmada Streptococcus mutans NCTC10449 susu, Tryptic Soy Broth (TSB)'da 24 saat üretildi ve koloni sayma metoduyla 1X109 CFU/ml olacak şekilde süspansiyon hazırlandı. Tryptic Soy Ağar (TSA) besiyeri yüzeyine 1X109 CFU/ml bakteri süspansiyonundan 100 ul ekildi ve ABF bond primeri ve diğer iki sistemin tek şişe ajanları steril kağıt disklere emdirildi ve ağara hafif basıyla yerleştirildi. Aerobik şartlarda 37 C'da 24 saat inkübasyonun ardından önlenim halkaları ölçüldü. Her bir materyal için test 3 kez tekrarlandı. Mikro sulandırma metodunda ise 100 ul TSB içerisine aynı bakteri süspansiyonundan ve primer ve bonding ajanlardan 10'ar ul eklendi, inkübasyondan (37 C'da 24 saat) sonra her bir çukur içeriği TSA besiyerine ekildi ve 24 saat sonra üreme yönünden incelendi. Bulgular: Tüm ajanların, disk difüzyon metodu ile yapılan ölçümlerinde antibakteriyel etkiye sahip oldukları saptandı. Ajanların önlenim halkalarının ortalama çaplarının kullanılan materyal gruplarına göre farklılığı Student-Newman-Keuls testi ile değerlendirildi ve gruplar arasında anlamlı farklılık saptandı (p0.05). Mikro sulandırma metodunda ise üremeye rastlanmadı. Sonuç: Bu çalışmada kullanılan tüm ajanların S.mutans'ın NCTC1010449 suşuna karşı antibakteriyel etkisinin olduğu belirlendi.
Objective: To evaluate antibacterial effects of three different dentin bonding systems [ABF Bond (Kuraray Japan), PQ1(Ultradent, USA) ve Stae (SDI, Australia)], disc diffusion method and micro dilution method were used in the study. Material and Method: A strain of Streptococcus mutans (NCTC10449) was used as a test organism. The strain Streptococcus mutans NCTC10449 was cultivated in Tryptic Soy Broth (TSB) at 37°C for 24 hours. Then, 109 CFU/ml suspension was prepared in TSB by Colony Counting Method. One hundred ul from the bacterial suspension given above (109 CFU/ml) was transferred to the plates of Tryptic Soy Agar (TSA). Each of the agents in 50ul quantities were absorbed to steril paper discs and they were embedded into the agar. Following incubation period (at 37 "C'for 24 hour), inhibition zones in mm were recorded. The test was made in triplicates. In micro dilution method, 10 ul of each primers and bonding agents as well as of the same concentration of original bacteria suspension (10s CFU/ml), were added into 100 ul of TSB. The microplates were incubated at 37 °C for 24. Then, presence of growing bacteria was evaluated. Results: The study revealed that all the agents used had antibacterial effects as evidenced by disc diffusion method. Differences between the average diameters of the agents were significant (Student-Newman-Keuls test) (p<0.05). Results from micro dilution method were found to be in parallel with those from disc diffusion method since no cultivation occured in the TSB. Conclusion: All tested agents were observed to have some antibacterial effects on S.mutans'm NCTC1010449 strain.
Objective: To evaluate antibacterial effects of three different dentin bonding systems [ABF Bond (Kuraray Japan), PQ1(Ultradent, USA) ve Stae (SDI, Australia)], disc diffusion method and micro dilution method were used in the study. Material and Method: A strain of Streptococcus mutans (NCTC10449) was used as a test organism. The strain Streptococcus mutans NCTC10449 was cultivated in Tryptic Soy Broth (TSB) at 37°C for 24 hours. Then, 109 CFU/ml suspension was prepared in TSB by Colony Counting Method. One hundred ul from the bacterial suspension given above (109 CFU/ml) was transferred to the plates of Tryptic Soy Agar (TSA). Each of the agents in 50ul quantities were absorbed to steril paper discs and they were embedded into the agar. Following incubation period (at 37 "C'for 24 hour), inhibition zones in mm were recorded. The test was made in triplicates. In micro dilution method, 10 ul of each primers and bonding agents as well as of the same concentration of original bacteria suspension (10s CFU/ml), were added into 100 ul of TSB. The microplates were incubated at 37 °C for 24. Then, presence of growing bacteria was evaluated. Results: The study revealed that all the agents used had antibacterial effects as evidenced by disc diffusion method. Differences between the average diameters of the agents were significant (Student-Newman-Keuls test) (p<0.05). Results from micro dilution method were found to be in parallel with those from disc diffusion method since no cultivation occured in the TSB. Conclusion: All tested agents were observed to have some antibacterial effects on S.mutans'm NCTC1010449 strain.
Açıklama
Anahtar Kelimeler
Diş Hekimliği
Kaynak
Gazi Üniversitesi Diş Hekimliği Fakültesi Dergisi (.Acta Odontologica Turcica)
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Scopus Q Değeri
Cilt
22
Sayı
1
