The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

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dc.contributor.authorErisen, Semiha
dc.contributor.authorAtalay, Emine
dc.contributor.authorYorgancilar, Mustafa
dc.date.accessioned2020-03-26T18:16:45Z
dc.date.available2020-03-26T18:16:45Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with alpha-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. The highest number of shoots, (15 per explant) was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). The rooted plantlets were acclimatised and produced normal plants.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-106O136]en_US
dc.description.sponsorshipThis research was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Project Number: TOVAG-106O136.en_US
dc.identifier.doi10.3906/bot-1009-74en_US
dc.identifier.endpage526en_US
dc.identifier.issn1300-008Xen_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage521en_US
dc.identifier.urihttps://dx.doi.org/10.3906/bot-1009-74
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26922
dc.identifier.volume35en_US
dc.identifier.wosWOS:000295501300004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF BOTANYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAstragalus cariensisen_US
dc.subjectendemicen_US
dc.subjectcallusen_US
dc.subjectorganogenesisen_US
dc.subjectthidiazuronen_US
dc.titleThe effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkeyen_US
dc.typeArticleen_US

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