Changes in the alternative electron sinks and antioxidant defence in chloroplasts of the extreme halophyte Eutrema parvulum (Thellungiella parvula) under salinity

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dc.contributor.authorUzilday, Baris
dc.contributor.authorOzgur, Rengin
dc.contributor.authorSekmen, A. Hediye
dc.contributor.authorYildiztugay, Evren
dc.contributor.authorTurkan, Ismail
dc.date.accessioned2020-03-26T19:01:15Z
dc.date.available2020-03-26T19:01:15Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground and Aims Eutrema parvulum (synonym, Thellungiella parvula) is an extreme halophyte that thrives in high salt concentrations (100-150 mM) and is closely related to Arabidopsis thaliana. The main aim of this study was to determine how E. parvulum uses reactive oxygen species (ROS) production, antioxidant systems and redox regulation of the electron transport system in chloroplasts to tolerate salinity. Methods Plants of E. parvulum were grown for 30 d and then treated with either 50, 200 or 300 mM NaCl. Physiological parameters including growth and water relationships were measured. Activities of antioxidant enzymes were determined in whole leaves and chloroplasts. In addition, expressions of chloroplastic redox components such as ferrodoxin thioredoxin reductases (FTR), NADPH thioredoxin reductases (NTRC), thioredoxins (TRXs) and peroxiredoxins (PRXs), as well as genes encoding enzymes of the water-water cycle and proline biosynthesis were measured. Key Results Salt treatment affected water relationships negatively and the accumulation of proline was increased by salinity. E. parvulum was able to tolerate 300 mM NaCl over long periods, as evidenced by H2O2 content and lipid peroxidation. While Ca2+ and K+ concentrations were decreased by salinity, Na+ and Cl-concentrations increased. Efficient induction of activities and expressions of water-water cycle enzymes might prevent accumulation of excess ROS in chloroplasts and therefore protect the photosynthetic machinery in E. parvulum. The redox homeostasis in chloroplasts might be achieved by efficient induction of expressions of redox regulatory enzymes such as FTR, NTRC, TRXs and PRXs under salinity. Conclusions E. parvulum was able to adapt to osmotic stress by an efficient osmotic adjustment mechanism involving proline and was able to regulate its ion homeostasis. In addition, efficient induction of water-water cycle enzymes and other redox regulatory components such as TRXs and PRXs in chloroplasts were able to protect the chloroplasts from salinity-induced oxidative stress.en_US
dc.identifier.doi10.1093/aob/mcu184en_US
dc.identifier.endpage463en_US
dc.identifier.issn0305-7364en_US
dc.identifier.issn1095-8290en_US
dc.identifier.issue3en_US
dc.identifier.pmid25231894en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage449en_US
dc.identifier.urihttps://dx.doi.org/10.1093/aob/mcu184
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31914
dc.identifier.volume115en_US
dc.identifier.wosWOS:000349560400011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOXFORD UNIV PRESSen_US
dc.relation.ispartofANNALS OF BOTANYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAlternative electron sinken_US
dc.subjectantioxidant enzymesen_US
dc.subjectchloroplastic redoxen_US
dc.subjectEutrema parvulumen_US
dc.subjecthalophyteen_US
dc.subjectoxidative stressen_US
dc.subjectperoxiredoxinen_US
dc.subjectplastid terminal oxidaseen_US
dc.subjectprolineen_US
dc.subjectsalinityen_US
dc.subjectThellungiella parvulaen_US
dc.subjectthioredoxinen_US
dc.subjectwater-water cycleen_US
dc.titleChanges in the alternative electron sinks and antioxidant defence in chloroplasts of the extreme halophyte Eutrema parvulum (Thellungiella parvula) under salinityen_US
dc.typeArticleen_US

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