Comparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigants

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dc.contributor.authorEldeniz, Ayce Unverdi
dc.contributor.authorGuneser, Mehmet Burak
dc.contributor.authorAkbulut, Makbule Bilge
dc.date.accessioned2020-03-26T19:01:26Z
dc.date.available2020-03-26T19:01:26Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.en_US
dc.description.sponsorshipSelcuk University, Scientific Research Project Coordination CenterSelcuk Universityen_US
dc.description.sponsorshipThis study was partially supported by a grant given by Selcuk University, Scientific Research Project Coordination Center and conducted in the Research Laboratories of Faculty of Dentistry. The authors thank Assoc. Prof. Coskun Kus for his generous help with the statistics.en_US
dc.identifier.doi10.1007/s10103-013-1387-1en_US
dc.identifier.endpage675en_US
dc.identifier.issn0268-8921en_US
dc.identifier.issn1435-604Xen_US
dc.identifier.issue2en_US
dc.identifier.pmid23884903en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage669en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10103-013-1387-1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31942
dc.identifier.volume30en_US
dc.identifier.wosWOS:000349549500025en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGER LONDON LTDen_US
dc.relation.ispartofLASERS IN MEDICAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectIrrigation solutionsen_US
dc.subjectCandida albicansen_US
dc.subjectOctenidineen_US
dc.subjectLight-activated disinfectionen_US
dc.subjectPhotodynamic therapyen_US
dc.subjectToluidine blueen_US
dc.titleComparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigantsen_US
dc.typeArticleen_US

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