Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis

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dc.contributor.authorKennedy, Sarah A.
dc.contributor.authorConstable, Peter D.
dc.contributor.authorSen, Ismail
dc.contributor.authorCouetil, Laurent
dc.date.accessioned2020-03-26T18:25:21Z
dc.date.available2020-03-26T18:25:21Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective-To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples. Sample-Blood samples obtained from 8 healthy horses. Procedures-Heparinized jugular venous blood was equilibrated via a tonometer at 37 degrees C with 12% O-2 and 5% CO2. Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0 degrees C) or at room temperature (22 degrees C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis. Results-Blood Po-2 remained constant for the reference method (GS stored at 0 degrees C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22 degrees C. In contrast, Po-2 increased when blood was stored at 0 degrees C in a GPPS and PSBGA or at 22 degrees C in a GPPS; however, Po-2 did not change when blood was stored at 22 degrees C in a PSBGA. Calculated values for plasma concentration of HCO3 and total CO2 concentration remained constant in the 3 syringe types when blood was stored at 22 degrees C for 2 hours but increased when blood was stored in a GS or GPPS at 0 degrees C. Conclusions and Clinical Relevance-Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0 degrees C or collected into a PSBGA and stored at room temperature. (Am J Vet Res 2012;73:979-987)en_US
dc.description.sponsorshipIndiana Horse Racing Commission; Purdue University College of Veterinary Medicine; Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipSupported in part by a grant from the Indiana Horse Racing Commission. Ms. Kennedy was supported in part by the Purdue University College of Veterinary Medicine 2010 Veterinary Scholars Summer Research Program. Dr. Sen was supported in part by The Scientific and Technical Research Council of Turkey.en_US
dc.identifier.doi10.2460/ajvr.73.7.979en_US
dc.identifier.endpage987en_US
dc.identifier.issn0002-9645en_US
dc.identifier.issn1943-5681en_US
dc.identifier.issue7en_US
dc.identifier.pmid22738049en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage979en_US
dc.identifier.urihttps://dx.doi.org/10.2460/ajvr.73.7.979
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27994
dc.identifier.volume73en_US
dc.identifier.wosWOS:000305669800009en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAMER VETERINARY MEDICAL ASSOCen_US
dc.relation.ispartofAMERICAN JOURNAL OF VETERINARY RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleEffects of syringe type and storage conditions on results of equine blood gas and acid-base analysisen_US
dc.typeArticleen_US

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