Effects of Mineral Trioxide Aggregate on Cell Survival, Gene Expression Associated with Mineralized Tissues, and Biomineralization of Cementoblasts

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dc.contributor.authorHakki, Sema S.
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorBelli, Sema
dc.date.accessioned2020-03-26T17:38:29Z
dc.date.available2020-03-26T17:38:29Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe purpose of this study was to investigate the effects of mineral trioxide aggregate (MTA) on survival, mineralization, and expression of mineralization-related genes of cementoblasts. Immortalized cementoblasts (OCCM) were maintained with Dulbecco modified Eagle medium containing 10% fetal bovine serum. Methyl-thiazol-diphenyl-tetrazolium experiments were performed at 24 and 72 hours to evaluate bioactive components released by MTA (0.002-20 mg/mL) on the cell survival of OCCM. Von Kossa staining was used to evaluate biomineralization of OCCM Cells. Images of cementoblasts were taken on day 3 by using inverted microscopy. Gene transcripts for bone sialoprotein (BSP), OCN, collagen type I (COL I), and osteopontin (OPN) were evaluated on days 3 and 5 by using semi-quantitative reverse transcriptase polymerase chain reaction. The 20 mg/mL concentration of MTA was toxic for OCCM cells, whereas other concentrations of MTA tested exhibited similar cell numbers when compared with control group, and the 0.02 mg/mL concentration of MTA increased OCCM cell survival at 72 hours. Although an apparent decrease n mineralization was observed in the highest 3 concentrations of MTA used, 0.02 and 0.002 mg/mL concentrations of MTA induced greater biomineralization of OCCM cells than seen in the control. Moreover, increased BSP and COL I mRNA expression was observed at 0.02 and 0.002 mg/mL concentrations of MTA. MTA did not have a negative effect on the viability and morphology of cementoblasts and induced biomineralization of cementoblasts at the concentrations of 0.02 and 0.002 mg/mL. Based on these results MTA can be considered as a favorable material regarding cell-material interaction. (J Endod 2009;35:513-519)en_US
dc.identifier.doi10.1016/j.joen.2008.12.016en_US
dc.identifier.endpage519en_US
dc.identifier.issn0099-2399en_US
dc.identifier.issue4en_US
dc.identifier.pmid19345796en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage513en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.joen.2008.12.016
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23492
dc.identifier.volume35en_US
dc.identifier.wosWOS:000265393000007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofJOURNAL OF ENDODONTICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCell viabilityen_US
dc.subjectcementoblastsen_US
dc.subjectmineral trioxide aggregateen_US
dc.subjectmineralizationen_US
dc.subjectmRNA expressionen_US
dc.titleEffects of Mineral Trioxide Aggregate on Cell Survival, Gene Expression Associated with Mineralized Tissues, and Biomineralization of Cementoblastsen_US
dc.typeArticleen_US

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