Determination of the effects of aflatoxin B-1 given in ovo on the proximal tibial growth plate of broiler chickens: histological, histometric and immunohistochemical findings

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dc.contributor.authorOznurlu, Y.
dc.contributor.authorCelik, I.
dc.contributor.authorSur, E.
dc.contributor.authorOzaydin, T.
dc.contributor.authorOguz, H.
dc.contributor.authorAltunbas, K.
dc.date.accessioned2020-03-26T18:24:46Z
dc.date.available2020-03-26T18:24:46Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractDetrimental effects of aflatoxin B-1 (AFB(1)) on the embryonic development of broiler tibia and its proximal growth plate were determined by means of histological, histometric and immunohistochemical methods. For this, 420 fertile eggs from parent stocks of Ross 308 broiler chickens were divided into five groups according to the proposed treatment: a control untreated group, a group injected with 30% ethanol and three further groups to be injected with 5, 15 or 40 ng AFB(1). The eggs were injected into the air space prior to incubation. Five eggs from each group were opened at 9, 11, 13, 17, 19 and 21 days of incubation and tibial tissue samples were removed, dissected of muscle and connective tissues, and processed by means of routine histological techniques. The cell proliferation rate of the epiphyseal growth plate cells was determined by immunohistochemical assay of proliferating cell nuclear antigen (PCNA) expression. The results showed that both proliferative and hypertrophic zones narrowed significantly (P<0.05), when compared with the controls, in all of the AFB(1)-treated groups whereas the transitional zone thickened, especially in the group given 40 ng AFB(1) group. The PCNA positivity indices of 15 and 40 ng AFB(1)-treated groups were significantly higher (P<0.05) on days 11, 13, 17, 19 and 21 of incubation. It was concluded that in ovo-administered AFB(1) adversely affected embryonic development of the tibial growth plate, and that affected hatched broilers might also be more susceptible to skeletal disorders during growth.en_US
dc.description.sponsorshipSUBAP "Scientific Research Projects Coordinating Office of Selcuk University"Selcuk University [08401069]en_US
dc.description.sponsorshipThis study was financed by SUBAP "Scientific Research Projects Coordinating Office of Selcuk University" (Project No. 08401069).en_US
dc.identifier.doi10.1080/03079457.2012.712673en_US
dc.identifier.endpage477en_US
dc.identifier.issn0307-9457en_US
dc.identifier.issn1465-3338en_US
dc.identifier.issue5en_US
dc.identifier.pmid22974164en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage469en_US
dc.identifier.urihttps://dx.doi.org/10.1080/03079457.2012.712673
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27904
dc.identifier.volume41en_US
dc.identifier.wosWOS:000309428000010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofAVIAN PATHOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleDetermination of the effects of aflatoxin B-1 given in ovo on the proximal tibial growth plate of broiler chickens: histological, histometric and immunohistochemical findingsen_US
dc.typeArticleen_US

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