The Identification of Genetic Variation in Insulin-Like Growth Factors-I (IGFI) Gene Region in Some Turkish Sheep Breeds
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Insulin-like growth factors (IGFs) areknown as peptides with important metabolic effects required for cellular growth and metabolism. IGF-I is synthesized in liver tissue under the control of growth hormone (GH) and released to blood. In the process of GH to accelerate growth, IGF-I occupies a large place. IGF-I clearly showed its important effects on the growth of animal studies. In this study, promoter region of the IGF-I gene were amplified by polymerase chain reaction (PCR) in sheep breeds (Akkaraman, Kivircik, Awassi, Sakiz, Daglıc, Morkaraman, MalyaKarayaka (15 to 20 sheep per breed)) reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with HaeII enzyme. The digestion of IGF-I gene with HaeII produced two alleles and three genotypes. Genotype frequencies were 59%, 19%and 22% for AA, BB and AB genotypes, respectively. Allele frequencies were 0.70 for A allele and 0.30 for the B allele. This study indicates the genetic profiles of the IGF-1 gene in native Turkish sheep breeds.
Açıklama
Anahtar Kelimeler
Growth hormone, Insulin-like growth factor (IGF), Sheep, Polymerase chain reaction (PCR, Restriction fragment length polymorphism (RFLP), Sheep breeds
Kaynak
Selcuk Journal of Agriculture and Food Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
3
Künye
Tawfeeq, M., Keskin, İ., İlhan, F., (2020). The Identification of Genetic Variation in Insulin-Like Growth Factors-I (IGFI) Gene Region in Some Turkish Sheep Breeds. Selcuk Journal of Agriculture and Food Sciences, 34(3), 189-192.
DOI: 10.15316/SJAFS.2020.215
