Utilization of the Ability to Induce Activation of the Nuclear Factor (Erythroid-derived 2)-like Factor 2 (Nrf2) to Assess Potential Cancer Chemopreventive Activity of Liquorice Samples

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dc.contributor.authorBasar, Norazah
dc.contributor.authorNahar, Lutfun
dc.contributor.authorOridupa, Olayinka Ayotunde
dc.contributor.authorRitchie, Kenneth J.
dc.contributor.authorTalukdar, Anupam D.
dc.contributor.authorStafford, Angela
dc.contributor.authorKushiev, Habibjon
dc.date.accessioned2020-03-26T19:31:49Z
dc.date.available2020-03-26T19:31:49Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroductionNuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) is a transcription factor that regulates expression of many detoxification enzymes. Nrf2-antioxidant responsive element (Nrf2-ARE) signalling pathway can be a target for cancer chemoprevention. Glycyrrhiza glabra, common name, liquorice', is used as a sweetening and flavouring agent, and traditionally, to treat various ailments, and implicated to chemoprevention. However, its chemopreventive property has not yet been scientifically substantiated. ObjectiveTo assess the ability of liquorice root samples to induce Nrf2 activation correlating to their potential chemopreventive property. MethodsThe ability of nine methanolic extracts of liquorice root samples, collected from various geographical origins, to induce Nrf2 activation was determined by the luciferase reporter assay using the ARE-reporter cell line, AREc32. The antioxidant properties were determined by the 2,2-diphenyl-1-picryhydrazyl (DPPH) and the ferric-reducing antioxidant power (FRAP) assays. ResultsAll extracts exhibited free-radical-scavenging property (RC50=136.39-635.66 mu g/mL). The reducing capacity of ferrous ion was 214.46-465.59M Fe(II)/g. Nrf2 activation indicated that all extracts induced expression of ARE-driven luciferase activity with a maximum induction of 2.3 fold relative to control. These activities varied for samples from one geographical location to another. ConclusionsThe present findings add to the existing knowledge of cancer chemoprevention by plant-derived extracts or purified phytochemicals, particularly the potential use of liquorice for this purpose. Copyright (c) 2016 John Wiley & Sons, Ltd. Nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) regulates expression of many detoxification enzymes, and Nrf2-antioxidant responsive element signalling pathway can be a target for cancer chemoprevention. The ability of liquorice root samples to induce Nrf2 activation correlating to their potential chemopreventive property was assessed by the luciferase reporter assay using the ARE-reporter cell line, AREc32. All extracts induced expression of ARE-driven luciferase activity, but this activity varied for samples from one geographical location to another.en_US
dc.description.sponsorshipMinistry of Education (MOE), Malaysiaen_US
dc.description.sponsorshipThe authors acknowledge the Ministry of Education (MOE), Malaysia, for financial support (NB) and thank Professor RolandWolf, University of Dundee, for provision of the AREc32 cell line.en_US
dc.identifier.doi10.1002/pca.2616en_US
dc.identifier.endpage238en_US
dc.identifier.issn0958-0344en_US
dc.identifier.issn1099-1565en_US
dc.identifier.issue5en_US
dc.identifier.pmid27527356en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage233en_US
dc.identifier.urihttps://dx.doi.org/10.1002/pca.2616
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34190
dc.identifier.volume27en_US
dc.identifier.wosWOS:000383448100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofPHYTOCHEMICAL ANALYSISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcancer chemopreventionen_US
dc.subjectnuclear factor (erythroid-derived 2)-like factor 2 (Nrf2)en_US
dc.subject2en_US
dc.subject2-diphenyl-1-picryhydrazyl (DPPH)en_US
dc.subjectferric-reducing antioxidant power (FRAP)en_US
dc.subjectGlycyrrhiza glabraen_US
dc.subjectFabaceaeen_US
dc.titleUtilization of the Ability to Induce Activation of the Nuclear Factor (Erythroid-derived 2)-like Factor 2 (Nrf2) to Assess Potential Cancer Chemopreventive Activity of Liquorice Samplesen_US
dc.typeArticleen_US

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