EFFECT OF VASOACTIVE INTESTINAL PEPTIDE (VIP) ON CYTOKINE LEVELS AND HAEMOSTATIC AND BIOCHEMICAL PARAMETERS IN A RAT ENDOTOXAEMIC MODEL
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
NATL VETERINARY RESEARCH INST
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The presented study was planned to determine whether vasoactive intestinal peptide (VIP) could prevent cytokine haemostatic, haematological, and biochemical disturbances in LPS-treated rats. Adult male Wistar rats (weight range: 200-250 g) were used. The study included four groups: group 1 served as a control group (C); animals in group 2 were given intravenously 1.6 mg/100 g of LPS (E. coli, serotype 0.111:B4); in group 3, rats were injected intraperitoneally with 25 ng/kg of VIP; in group 4, the same doses of VIP and I,PS were injected simultaneously. Blood samples were collected 6 h after treatments. In endotoxaemic rats, platelet count, fibrinogen, and antithrombin levels were decreased, the activated partial thromboplastin time and prothrombin time were prolonged, and leucopoenia, as well as significant changes in differential leukocyte percentage were demonstrated. In addition, LPS caused statistically significant increases in plasma INF-alpha, IL-6, and IL-10 levels, and AST, ALT, creatinine, cholesterol, triglyceride concentrations. However, it caused a statistically significant decrease in total protein and albumin levels when compared to control group. The results showed that during endotoxaemia, VIP had moderately therapeutic effect as an antiinflammatory agent, suppressing INF-alpha and IL-6, and stimulating IL-10; however, it was not effective against the adverse effect of LPS on investigated haematological and biochemical parameters.
Açıklama
Anahtar Kelimeler
rat, endotoxin, vasoactive intestinal peptide, cytokine, disseminated intravascular coagulation
Kaynak
BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
56
Sayı
1