EFFECT OF VASOACTIVE INTESTINAL PEPTIDE (VIP) ON CYTOKINE LEVELS AND HAEMOSTATIC AND BIOCHEMICAL PARAMETERS IN A RAT ENDOTOXAEMIC MODEL

Küçük Resim Yok

Tarih

2012

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

NATL VETERINARY RESEARCH INST

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

The presented study was planned to determine whether vasoactive intestinal peptide (VIP) could prevent cytokine haemostatic, haematological, and biochemical disturbances in LPS-treated rats. Adult male Wistar rats (weight range: 200-250 g) were used. The study included four groups: group 1 served as a control group (C); animals in group 2 were given intravenously 1.6 mg/100 g of LPS (E. coli, serotype 0.111:B4); in group 3, rats were injected intraperitoneally with 25 ng/kg of VIP; in group 4, the same doses of VIP and I,PS were injected simultaneously. Blood samples were collected 6 h after treatments. In endotoxaemic rats, platelet count, fibrinogen, and antithrombin levels were decreased, the activated partial thromboplastin time and prothrombin time were prolonged, and leucopoenia, as well as significant changes in differential leukocyte percentage were demonstrated. In addition, LPS caused statistically significant increases in plasma INF-alpha, IL-6, and IL-10 levels, and AST, ALT, creatinine, cholesterol, triglyceride concentrations. However, it caused a statistically significant decrease in total protein and albumin levels when compared to control group. The results showed that during endotoxaemia, VIP had moderately therapeutic effect as an antiinflammatory agent, suppressing INF-alpha and IL-6, and stimulating IL-10; however, it was not effective against the adverse effect of LPS on investigated haematological and biochemical parameters.

Açıklama

Anahtar Kelimeler

rat, endotoxin, vasoactive intestinal peptide, cytokine, disseminated intravascular coagulation

Kaynak

BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY

WoS Q Değeri

Q3

Scopus Q Değeri

N/A

Cilt

56

Sayı

1

Künye