Comparison of Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry Methods in the Measurement of Serum Androstenedione Levels

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dc.contributor.authorYücel, Kamile
dc.contributor.authorAbuşoğlu, Sedat
dc.contributor.authorÜnlü, Ali
dc.date.accessioned2020-03-26T19:53:11Z
dc.date.available2020-03-26T19:53:11Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. In this study, a new liquid chromatography- tandem mass spectrometry (LC-MS/MS) method was developed according to CLSI rules. The developed method was compared with two immunoassay methods, the enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Methods: After liquid-liquid extraction, a Shimadzu Prominence LC unit coupled to an ABSCIEX API 3200 mass spectrometer with atmospheric pressure chemical ionization was used to quantify serum androstenedione levels. Serum androstenedione results taken from tandem mass spectrometry were compared with the immunoassays. Results: The androstenedione assay was linear up to 50 ng/mL. Lower limit of quantitation and lower limit of detection were 0.195 ng/mL and 0.097 ng/mL, respectively. This method was not affected by matrix effect and other steroid hormone interferences. In this study, the obtained recovery was 69 - 99%, carryover value was determined as 0.026 ng/mL. According to the results of an interference study, androstenedione bias % did not exceed the limit of allowable bias % and 88.7% recovery was acquired for androstenedione. In the LC-MS/MS and ELISA comparison study, the slope value was found as 18.412, intercept -22.87, and r(2) value as 0.1033. In the LC-MS/MS and RIA comparison study, slope value was found as 1.085, intercept 0.4541, and r(2) value as 0.3712. In the RIA and ELISA comparison study, slope value was found as 9.57, intercept -15.5, and r(2) value as 0.19. Conclusions: The LC-MS/MS provides agreement with the results of radioimmunoassay but not with ELISA. This method offers better selectivity compared to immunoassay systems.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinator [13202 041]en_US
dc.description.sponsorshipNo conflicts of interest are associated with this study. This research was supported by Selcuk University Scientific Research Projects Coordinator (Proj. No.: 13202 041).en_US
dc.identifier.doi10.7754/Clin.Lab.2017.170612en_US
dc.identifier.endpage75en_US
dc.identifier.issn1433-6510en_US
dc.identifier.issue01.02.2020en_US
dc.identifier.pmid29479885en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage69en_US
dc.identifier.urihttps://dx.doi.org/10.7754/Clin.Lab.2017.170612
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36428
dc.identifier.volume64en_US
dc.identifier.wosWOS:000428712600009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCLIN LAB PUBLen_US
dc.relation.ispartofCLINICAL LABORATORYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectandrostenedioneen_US
dc.subjectmethod validationen_US
dc.subjectLC-MS/MSen_US
dc.subjectimmunoassayen_US
dc.titleComparison of Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry Methods in the Measurement of Serum Androstenedione Levelsen_US
dc.typeArticleen_US

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