Evaluation of new silica-based humic acid stationary phase for the separation of tocopherols in cold-pressed oils by normal-phase high-performance liquid chromatography

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dc.contributor.authorAyyildiz, Hamide Filiz
dc.date.accessioned2020-03-26T19:05:57Z
dc.date.available2020-03-26T19:05:57Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA new humic acid stationary phase was prepared by immobilizing humic acid onto aminopropyl silica via an amide linkage formation and used, for the first time, for the separation and quantification of the tocopherol compounds in cold-pressed oil samples under normalphase high-performance liquid chromatography conditions. Parameters affecting the chromatographic separation such as mobile phase composition and flow rate were optimized. By evaluating the calculations of capacity factor, asymmetry factor, resolution, selectivity factor, and theoretical plate number, the best separation was obtained with isocratic elution of n-hexane and isopropyl alcohol (99:1% v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector set at excitation and emission wavelengths 295 and 330 nm, respectively. All compounds were separated in 20 min. The method was validated according to international guidelines and found to be linear in a wide concentration range, also the mean recovery of the compounds ranged from 97.9 to 99.2%, with a CV less than 2.7% in all cases. The results showed that the developed stationary phase is suitable for the separation and quantification of the tocopherol compounds in real oil samples.en_US
dc.description.sponsorshipSelcuk University Coordination of Scientific ResearchSelcuk University [SU-BAP 14401096]en_US
dc.description.sponsorshipThe author would like to thank Selcuk University Coordination of Scientific Research for supporting research with SU-BAP 14401096 project number.en_US
dc.identifier.doi10.1002/jssc.201401377en_US
dc.identifier.endpage820en_US
dc.identifier.issn1615-9306en_US
dc.identifier.issn1615-9314en_US
dc.identifier.issue5en_US
dc.identifier.pmid25557082en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage813en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jssc.201401377
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32133
dc.identifier.volume38en_US
dc.identifier.wosWOS:000350201900014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.relation.ispartofJOURNAL OF SEPARATION SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHumic aciden_US
dc.subjectImmobilizationen_US
dc.subjectNormal-phase high-performance liquid chromatographyen_US
dc.subjectStationary phaseen_US
dc.subjectTocopherolen_US
dc.titleEvaluation of new silica-based humic acid stationary phase for the separation of tocopherols in cold-pressed oils by normal-phase high-performance liquid chromatographyen_US
dc.typeArticleen_US

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