Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera

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dc.contributor.authorYeh, Hung-Yueh
dc.contributor.authorTelli, Arife Ezgi
dc.contributor.authorJagne, Jarra F.
dc.contributor.authorBenson, Christopher L., II
dc.contributor.authorHiett, Kelli L.
dc.contributor.authorLine, John E.
dc.date.accessioned2020-03-26T19:24:00Z
dc.date.available2020-03-26T19:24:00Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCampylobacter jejuni, a Gram-negative rod, is a zoonotic pathogen associated with human acute bacterial gastroenteritis worldwide. The flagellum, composed of more than 35 proteins, is responsible for colonization of C. jejuni in the host gastrointestinal tract as well as inducing protective antibodies against the homologous serotype. In our previous study, we demonstrated that the flagellar capping protein (FliD) is an immunodominant protein that reacted strongly to sera from field chickens. In this communication, we mapped linear immunoreactive epitopes on FliD using a set of 158 synthetic peptides of 15-mer overlapping with 11 amino acid residues on peptide microarrays with sera from field chickens. The results from peptide microarrays showed (1) no cross-reactivity of the immobilized peptides with the secondary anti-chicken antibody in the control incubation, and (2) heterogeneous patterns of sera reacting to the immobilized peptides. The peptides that reacted to more than three chicken sera and had higher averages of fluorescence units were selected for further validation by the peptide ELISA. The results showed peptides 24, 91 and 92 had relatively high reactivity and less variation among 64 individual serum samples, indicating these peptides represented the shared immunodominant epitopes on the C. jejuni FliD protein. These peptides were also recognized by sera from chickens immunized with the purified recombinant FliD protein. The findings of the specific shared linear immunodominant epitopes on FliD in this study provide a rationale for further evaluation to determine their utility as epitope vaccines covering multiple serotypes for chicken immunization, and subsequently, for providing safer poultry products for human consumption. Published by Elsevier Ltd.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); USDA Agricultural Research Service CRIS Project [6612-32000-060-00]; U.S. Poultry & Egg Association Project [679]en_US
dc.description.sponsorshipWe thank Susan Q. Brooks of the Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA for technical assistance. We are grateful to Drs. Jaxk Reeves and Yan Du of the Department of Statistics, the University of Georgia, Athens, GA for statistical analysis. Arife Ezgi Telli was a visiting scholar supported by The Scientific and Technological Research Council of Turkey. Christopher L. Benson II was the Peach State Louis Stokes Alliance for Minority Participation (LSAMP) Research Scholar from The University of Georgia. This study was supported by the USDA Agricultural Research Service CRIS Project No. 6612-32000-060-00 and the U.S. Poultry & Egg Association Project No. 679. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture, which is an equal opportunity provider and employer.en_US
dc.identifier.doi10.1016/j.cimid.2016.10.003en_US
dc.identifier.endpage81en_US
dc.identifier.issn0147-9571en_US
dc.identifier.issn1878-1667en_US
dc.identifier.pmid27865268en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage76en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cimid.2016.10.003
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33553
dc.identifier.volume49en_US
dc.identifier.wosWOS:000390521800010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTDen_US
dc.relation.ispartofCOMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCampylobacter jejunien_US
dc.subjectFlagellar capping proteinen_US
dc.subjectFliDen_US
dc.subjectEpitope mappingen_US
dc.subjectZoonosisen_US
dc.subjectFoodborne pathogenen_US
dc.titleEpitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) seraen_US
dc.typeArticleen_US

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