Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey

Basit Öğe Kaydı
dc.contributor.authorZhou, Mo
dc.contributor.authorCao, Shinuo
dc.contributor.authorSevinc, Ferda
dc.contributor.authorSevinc, Mutlu
dc.contributor.authorCeylan, Onur
dc.contributor.authorMoumouni, Paul Franck Adjou
dc.contributor.authorJirapattharasate, Charoonluk
dc.date.accessioned2020-03-26T19:25:10Z
dc.date.available2020-03-26T19:25:10Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBabesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in cattle from 6 provinces of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. bigemina rhoptry-associated protein la (BbiRAP-1a), T. annulata merozoite surface antigen-1 (Tams-1), T. orientalis major piroplasm surface protein (ToMPSP) and A. marginale major surface protein 4 (AmMSP4) genes, respectively. Fragments of B. bigemina internal transcribed spacer (BbiITS), T. annulata internal transcribed spacer (TaITS),ToMPSP and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the overall infections of A. marginale, T. annulata, B. bigemina and T. orientalis were 29.1%, 18.9%, 11.2% and 5.6%, respectively. The co-infection of two or three pathogens was detected in 29/196 (15.1%) of the cattle samples. The results of sequence analysis indicated that BbiRAP-1a, BbiITS, Tams-1, ToMPSP and AmMSP4 were conserved among the Turkish samples, with 99.76%, 99-99.8%, 99.34-99.78%, 96.9-99.61% and 99.42-99.71% sequence identity values, respectively. In contrast, the Turkish TaITS gene sequences were relatively diverse with 92.3-96.63% identity values. B. bigemina isolates from Turkey were found in the same clade as the isolates from other countries in phylogenetic analysis. On the other hand, phylogenetic analysis based on T. annulata ITS sequences revealed significant differences in the genotypes of T. annulata isolates from Turkey. Additionally, the T. orientalis isolates from Turkish samples were classified as MPSP type 3 genotype. This is the first report of type 3 MPSP in Turkey. Moreover, AmMSP4 isolates from Turkey were found in the same clade as the isolates from other countries. This study provides important data for understanding the epidemiology of tick-borne diseases and it is expected to improve approach for diagnosis and control of tick-borne diseases in Turkey. (C) 2015 Elsevier GmbH. All rights reserved.en_US
dc.description.sponsorshipMinistry of Education, Culture, Sports, Science, and Technology, JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-113-O-336]en_US
dc.description.sponsorshipThis work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan, and Technological Research Council of Turkey (TUBITAK), TOVAG-113-O-336.en_US
dc.identifier.doi10.1016/j.ttbdis.2015.09.008en_US
dc.identifier.endpage134en_US
dc.identifier.issn1877-959Xen_US
dc.identifier.issn1877-9603en_US
dc.identifier.issue1en_US
dc.identifier.pmid26492823en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage126en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ttbdis.2015.09.008
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33796
dc.identifier.volume7en_US
dc.identifier.wosWOS:000366953400019en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER GMBHen_US
dc.relation.ispartofTICKS AND TICK-BORNE DISEASESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTick-borne pathogenen_US
dc.subjectSpecies-specific PCRen_US
dc.subjectCattleen_US
dc.subjectTurkeyen_US
dc.titleMolecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkeyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu