Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy

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dc.contributor.authorAtli, Mehmet Osman
dc.contributor.authorGuzeloglu, Aydin
dc.contributor.authorDinc, Dursun Ali
dc.date.accessioned2020-03-26T18:14:39Z
dc.date.available2020-03-26T18:14:39Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractWNT signaling pathway plays important roles in reproductive events. Aims were to (1) determine presence of WNT genes and their antagonists in equine endometrium; and (2) to evaluate their expression profiles during early pregnancy. Endometrial biopsies were obtained from mares on day of ovulation (d0, n = 4) and on days of 14 (P14, n = 4), 18 (P18, n = 4), 22 (P22, n = 4) of early pregnancy. Biopsies were also collected from cyclic mares during late diestrus (LD, on day of 13.5-14, n = 4) and after luteolysis in estrus phase (AL on day of 17.5-18, is = 4) of the cycle. PCR was used to detect expression of genes studied and then relative expression levels were quantified using real-time PCR analysis. A mixed model was fitted on the normalized data and least significant difference test (alpha = 0.05) was employed. Eleven WNT genes (WNT2, WNT2B, WNT4, WNT5A, WNT5B, WNT7A, WNT8A, WNT9B, WNT10B, WNT11 and WNT16) and their antagonists (SFRP1, SFRP2, SFRP5, DKK1, DKK2 and WIF-1) were detected in equine endometrium. Compared to d0, WNT2, WNT5B, WNT7A and SFRP1 expressions were downregulated by the pregnancy while DKK1 was upregulated. WNT5A, WNT11 and WIF-1 were upregulated on P14 and P18. but WNT2B increased only on P14. When LD and P14 were compared, level of WNT8A decreased on P14 while increase in WNT4 level on P14 was slightly significant (P < 0.06). Levels of WNT7A and SFRP1 decreased while DKK1 and WIF-1 increased by the pregnancy on P18 compared to AL Moreover, WNT2B, WNT5A, WNT9B, WNT10B, WNT11, WNT16 DKK1 and WIF-1 were upregulated on LD compared to AL whereas WNT4, WNT7A, SFRP1 were downregulated. In conclusion, the results demonstrate that WNT genes and their antagonists appear to be regulated during early pregnancy in equine endometrium possibly due to embryonic factors and/or maternal progesterone. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSelcuk UniversitySelcuk University [SUBAP 08202002]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 107O035]en_US
dc.description.sponsorshipThis study was partially supported by grants from Selcuk University Scientific Research Project (grant SUBAP 08202002) and The Scientific and Technological Research Council of Turkey (TUBITAK) grant TOVAG 107O035 (to AG). The authors would like to thank Dr. Seyit A. Kayis for assistance in statistical analysis and Dr. Ercan Kurar and Dr. Ahmet Semacan for assistance in laboratory and field studies.en_US
dc.identifier.doi10.1016/j.anireprosci.2011.04.001en_US
dc.identifier.endpage102en_US
dc.identifier.issn0378-4320en_US
dc.identifier.issue01.04.2020en_US
dc.identifier.pmid21550190en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage94en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.anireprosci.2011.04.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26487
dc.identifier.volume125en_US
dc.identifier.wosWOS:000292668800013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofANIMAL REPRODUCTION SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectWNTen_US
dc.subjectEquineen_US
dc.subjectEndometriumen_US
dc.subjectPregnancyen_US
dc.titleExpression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancyen_US
dc.typeArticleen_US

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