Immobilization of albumin on indium-tin oxide (ITO) surface via isocyanate linkage
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE SA
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Indium-tin oxide (ITO) surfaces have become a very important material for use as a conducting substrate, recently. Immobilization of biological molecule onto the ITO is a fundamental step in the development of biosensors. In this paper, attachment of albumin was carried out using ITO as a support. Albumin was covalently immobilized onto self-assembled monolayer (SAM) of (3-isocyanatopropyl) triethoxysilane (IPTES) on ITO surface. Isocyanato-functionalized organic film was prepared by self-assembling on active ITO surface. After that, human serum albumin (HAS) which was prepared in Phosphate-Buffered Saline Solution (PBS: pH 7.4, 0.1 M), was covalently immobilized on IPTES modified ITO surface. The study here can help us understand the influence of monolayer and electrochemical behavior on ITO surface. Characterization of stepwise changes of active ITO, IPTES/ITO and albumin/IPTES/ITO surfaces were carried out using contact angle (CA) measurements, fluorescence microscopy, atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. (C) 2009 Elsevier B.V. All rights reserved
Açıklama
Anahtar Kelimeler
Indium-tin oxide, Self-assembled monolayer, Albumin immobilization, Electrochemical impedance, Atomic force microscopy
Kaynak
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
633
Sayı
1
