Immobilization of albumin on indium-tin oxide (ITO) surface via isocyanate linkage

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dc.contributor.authorÖzmen, Mustafa
dc.contributor.authorCan, Keziban
dc.contributor.authorErsöz, Mustafa
dc.date.accessioned2020-03-26T17:38:53Z
dc.date.available2020-03-26T17:38:53Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIndium-tin oxide (ITO) surfaces have become a very important material for use as a conducting substrate, recently. Immobilization of biological molecule onto the ITO is a fundamental step in the development of biosensors. In this paper, attachment of albumin was carried out using ITO as a support. Albumin was covalently immobilized onto self-assembled monolayer (SAM) of (3-isocyanatopropyl) triethoxysilane (IPTES) on ITO surface. Isocyanato-functionalized organic film was prepared by self-assembling on active ITO surface. After that, human serum albumin (HAS) which was prepared in Phosphate-Buffered Saline Solution (PBS: pH 7.4, 0.1 M), was covalently immobilized on IPTES modified ITO surface. The study here can help us understand the influence of monolayer and electrochemical behavior on ITO surface. Characterization of stepwise changes of active ITO, IPTES/ITO and albumin/IPTES/ITO surfaces were carried out using contact angle (CA) measurements, fluorescence microscopy, atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. (C) 2009 Elsevier B.V. All rights reserveden_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-U/182, 106T718]en_US
dc.description.sponsorshipWe would like to thank Dr. Yasemin Oztekin for helping electrochemical impedance measurements in her laboratory. This work was carried out during the framework of Can, K. master degree prograrn at University of Selcuk, Turkey. This work was financially supported by the Scientific and Technological Research Council of Turkey Projects (TBAG-U/182, 106T718).en_US
dc.identifier.doi10.1016/j.jelechem.2009.06.004en_US
dc.identifier.endpage234en_US
dc.identifier.issn1572-6657en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage228en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jelechem.2009.06.004
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23598
dc.identifier.volume633en_US
dc.identifier.wosWOS:000268938900031en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE SAen_US
dc.relation.ispartofJOURNAL OF ELECTROANALYTICAL CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectIndium-tin oxideen_US
dc.subjectSelf-assembled monolayeren_US
dc.subjectAlbumin immobilizationen_US
dc.subjectElectrochemical impedanceen_US
dc.subjectAtomic force microscopyen_US
dc.titleImmobilization of albumin on indium-tin oxide (ITO) surface via isocyanate linkageen_US
dc.typeArticleen_US

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