Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

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dc.contributor.authorKars, Meltem Demirel
dc.contributor.authorKara, Reyhan
dc.contributor.authorGundogdu, Yasemin
dc.contributor.authorKepceoglu, Abdullah
dc.contributor.authorKilic, Hamdi Sukur
dc.date.accessioned2020-03-26T18:50:49Z
dc.date.available2020-03-26T18:50:49Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPurpose: Photodynamic therapy (PDT) is a type of photo-chemotherapy that is based on the application of photosensitizer and irradiation of the region by laser sources. Photosensitizer and light interaction will develop reactive oxygen radicals ( O-1(2)) in the cells and elimination of cells by apoptosis or necrosis. Methods: Metastatic skin cancer cells SKMEL-30 were treated by 5-ALA in dark and then they were irradiated by 90-femtosecond (fs) laser with different pulse powers for different durations. The effects of 5-ALA mediated photodynamic therapy on the cells were determined by XTT proliferation kit and by flow cytometry measurements of Annexin V, 7-AAD and mitochondrial membrane potential alterations. Fluorescent accumulation of protoporphyrin IX was investigated by fluorometry and confocal laser microscope. Results: The viability tests for SKMEL-30 cells treated with different 5-ALA doses and femtosecond laser power and durations demonstrated that 635 nm, 45 mW pulse energy at 90 fs laser pulse applications for 60 sec to 1 mM 5-ALA exposed cells decreased the cell proliferation by 30%. Flow cytometric measurements exhibit that PDT caused 63% of mitochondria membrane potential alteration, 30% of cell death in the population by apoptosis and 39% of cells by necrosis. There was 1 mM 5-ALA exposure that also exhibited about 32% accumulation of fluorescence in the cells. Conclusion: The pretreatment of the cells with the precursor 5-ALA lets the imaging due to increased protoporphyrin IX fluorescence. This treatment method may be proposed as an effective theranostic strategy for melanoma because of its rapid and effective anticancer consequences. (C) 2014 Elsevier Masson SAS. All rights reserved.en_US
dc.description.sponsorshipTurkish Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T523]; Selcuk University Research FundSelcuk University [DPT_2009K121080]en_US
dc.description.sponsorshipThis study was supported by theTurkish Research Council with the project number 111T523 and by Selcuk University Research Fund with the project number DPT_2009K121080.en_US
dc.identifier.doi10.1016/j.biopha.2014.04.001en_US
dc.identifier.endpage662en_US
dc.identifier.issn0753-3322en_US
dc.identifier.issn1950-6007en_US
dc.identifier.issue5en_US
dc.identifier.pmid24835696en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage657en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.biopha.2014.04.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30877
dc.identifier.volume68en_US
dc.identifier.wosWOS:000342667800021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIERen_US
dc.relation.ispartofBIOMEDICINE & PHARMACOTHERAPYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPhotodynamic therapyen_US
dc.subjectMelanomaen_US
dc.subjectApoptosisen_US
dc.subjectMitochondria membrane potentialen_US
dc.subjectFemtosecond laseren_US
dc.titleFemtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanomaen_US
dc.typeArticleen_US

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