Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability

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dc.contributor.authorSariozkan, Serpil
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorTuncer, Purhan B.
dc.contributor.authorBuyukleblebici, Serhat
dc.contributor.authorCanturk, Fazile
dc.date.accessioned2020-03-26T18:51:08Z
dc.date.available2020-03-26T18:51:08Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSupplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 +/- 0.24% and 4.80 +/- 0.20%), glutamine (2.80 +/- 0.20% and 6.40 +/- 0.40%), carnitine (2.60 +/- 0.24% and 6.00 +/- 0.63%) and methionine (3.40 +/- 0.51% and 9.20 +/- 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 +/- 0.44 and 15.60 +/- 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 +/- 1.70% and 62.40 +/- 1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 +/- 4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2 mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17 +/- 0.18%) lower than those of the control group (3.16 +/- 0.32%) (P < 0.001). For pregnancy rates, there were no significant differences among the extender groups (P > 0.05). Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserveden_US
dc.identifier.doi10.1016/j.cryobiol.2014.01.007en_US
dc.identifier.endpage133en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue1en_US
dc.identifier.pmid24468272en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage129en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2014.01.007
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30930
dc.identifier.volume68en_US
dc.identifier.wosWOS:000332190100018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBovineen_US
dc.subjectAntioxidantsen_US
dc.subjectSperm parametersen_US
dc.subjectDNA damageen_US
dc.subjectFreeze-thawingen_US
dc.titleInfluence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing abilityen_US
dc.typeArticleen_US

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