Periodontal ligament cell behavior on different titanium surfaces

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dc.contributor.authorHakki, Sema S.
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorPurali, Nuhan
dc.contributor.authorKorkusuz, Feza
dc.contributor.authorBozkurt, Buket S.
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorOnder, M. Ercument
dc.date.accessioned2020-03-26T18:42:51Z
dc.date.available2020-03-26T18:42:51Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAim. The purpose of this study was to investigate proliferation, morphology, mineralization and mRNA expressions of mineralized tissue associated proteins of PDL cells on smooth (S), sandblasted small-grit (SSG), sandblasted large-grit (SLG) and sodium titanate (NaTi) coated titanium alloys, in vitro. Methods and materials: PDL cells were cultured with DMEM media containing 10% FBS on the S, SSG, SLG and NaTi titanium surfaces. PDL cell proliferation, mineralization and immunohistochemistry experiments for Bone Sialoprotein (BSP) were performed. The morphology of the PDL cells was examined using confocal and scanning electron microscopy (SEM). Gene expression profiles of cells were evaluated using a quantitative-polymerase chain reaction (Q-PCR) for type I collagen (COL I), Osteocalcin (OCN), osteopontin (OPN) and Runt-related transcription factor-2 (Runx2) on days 7 and 14. Results. Proliferation results on days 6 and 10 were similar in groups, while those of day 13 revealed a decrease in the NaTi group when compared to the S group. NaTi surface induced BSP mRNA expression which was correlated with mineralization tests and BSP immunostaining results. Increased Runx2 mRNA expression was also noted in the NaTi surface when compared to other surfaces. Conclusions. This study considers the NaTi surface as a potential alternative to SSG and SLG surfaces. This surface might provide a promising environment for PDL ligament-anchored implants.en_US
dc.description.sponsorshipTurkish Scientific Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-SBAG/3130]en_US
dc.description.sponsorshipThis study was performed at the Research Laboratory of Faculty of Dentistry, Selcuk University and was supported by Turkish Scientific Research Council (TUBITAK-SBAG/3130).en_US
dc.identifier.doi10.3109/00016357.2012.734417en_US
dc.identifier.endpage916en_US
dc.identifier.issn0001-6357en_US
dc.identifier.issn1502-3850en_US
dc.identifier.issue03.04.2020en_US
dc.identifier.pmid23088753en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage906en_US
dc.identifier.urihttps://dx.doi.org/10.3109/00016357.2012.734417
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29719
dc.identifier.volume71en_US
dc.identifier.wosWOS:000322832200078en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofACTA ODONTOLOGICA SCANDINAVICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjecttitanium surfaceen_US
dc.subjectsodium titanate coatingen_US
dc.subjectimplanten_US
dc.subjectperiodontal ligament cellsen_US
dc.subjectscanning electron microscopyen_US
dc.subjectconfocal microscopyen_US
dc.subjectproliferationen_US
dc.subjectmineralizationen_US
dc.subjectmRNA expressionsen_US
dc.titlePeriodontal ligament cell behavior on different titanium surfacesen_US
dc.typeArticleen_US

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