Determination of ciprofloxacin and levofloxacin in human sputum collected from cystic fibrosis patients using microextraction by packed sorbent-high performance liquid chromatography photodiode array detector

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dc.contributor.authorLocatelli, Marcello
dc.contributor.authorCiavarella, Maria Teresa
dc.contributor.authorPaolino, Donatella
dc.contributor.authorCelia, Christian
dc.contributor.authorFiscarelli, Ersilia
dc.contributor.authorRicciotti, Gabriella
dc.contributor.authorPompilio, Arianna
dc.date.accessioned2020-03-26T19:01:46Z
dc.date.available2020-03-26T19:01:46Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis paper reports a new, easy, cheap, and fast MEPS-HPLC-PDA method for the simultaneous analysis of ciprofloxacin and levofloxacin, two fluoroquinolones (FLQs) commonly used for the treatment of pulmonary infections in cystic fibrosis (CF) patients. The FLQs were resolved on a Discovery C-8 column (250 mm x 4.6 mm; 5 mu m particle size) using an isocratic elution with a run time of 15 min, without further purification. The method was validated over concentrations ranging from 0.05 to 2 mu g/mL for both analytes in human sputum, and enrofloxacin was used as internal standard. This method was successfully tested to detect FLQs in sputum collected from CF patients. The MEPS-HPLC-PDA method was validated using biological samples collected from CF patients orally or intravenously injected with FLQs. The resultant data showed that the method is selective, sensitive and robust over range of concentrations for both FLQs. The limit of quantification of the method was 0.05 mu g/mL for both analytes (comparable to more complex and expensive instrument configurations), weighted-matrix-matched standard curves showed a good linearity up to 2 mu g/mL, and parallelism tests were also successfully assessed. The intra- and inter-day precision (RSD%) values were <= 10.4% and <= 11.1%, respectively, for all range of analysis. The intra- and inter-day trueness (Bias%) values are ranged from -11.8% to 7.25% for both antibiotic drugs. At the best of our knowledge, this is the first MEPS-HPLC-PDA based method that uses MEPS procedure for simultaneous determination of ciprofloxacin and levofloxacin in human sputum. The method was tested successfully on real sputum samples by following a conventional drug administration. Furthermore, the MEPS-HPLC-PDA based method provides more advantages to detect and analyze quickly the antibiotic drugs in biological matrices than other analytical procedures reported in literature. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipUniversity of Chieti - Pescara "G. d'Annunzio"en_US
dc.description.sponsorshipThis work was supported from grant ex 60%, University of Chieti - Pescara "G. d'Annunzio".en_US
dc.identifier.doi10.1016/j.chroma.2015.09.075en_US
dc.identifier.endpage66en_US
dc.identifier.issn0021-9673en_US
dc.identifier.issn1873-3778en_US
dc.identifier.pmid26431855en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage58en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.chroma.2015.09.075
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31993
dc.identifier.volume1419en_US
dc.identifier.wosWOS:000363826600007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofJOURNAL OF CHROMATOGRAPHY Aen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMEPS-HPLC-PDAen_US
dc.subjectMethod developmenten_US
dc.subjectLevofloxacin and ciprofloxacinen_US
dc.subjectSputumen_US
dc.subjectSample preparationen_US
dc.subjectCystic fibrosisen_US
dc.titleDetermination of ciprofloxacin and levofloxacin in human sputum collected from cystic fibrosis patients using microextraction by packed sorbent-high performance liquid chromatography photodiode array detectoren_US
dc.typeArticleen_US

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