Investigation of chemical profile, biological properties of Lotus corniculatus L. extracts and their apoptotic-autophagic effects on breast cancer cells

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dc.contributor.authorYerlikaya, Şerife
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorDiuzheva, Alina
dc.contributor.authorJeko, Jozsef
dc.contributor.authorCziaky, Zoltan
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T20:14:50Z
dc.date.available2020-03-26T20:14:50Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThis study aimed to reveal chemical profiles and biological activities of ethyl acetate (EA), methanol (MeOH), and water extracts of Lotus corniculatus. Ethnobotanical reports have indicated the importance of phytochemical properties of the genus Lotus. In this study, the effects of medicinal plant extracts on antioxidant (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays), enzyme inhibitory (on cholinesterase, tyrosinase, a-amylase and a-glucosidase), DNA protection and anticancer properties (including anti-proliferative, cell death and telomerase activity marker gene analysis, apoptotic DNA fragmentation analysis, cell migration test) were evaluated. According to chemical analysis, quercetin derivatives geraldol, isorhamnetin and kaempferol-O-coumaroylhexoside-O-deoxyhexoside isomers were dominant in the extracts. MeOH extracts showed the highest total flavonoids capacity with 21.13 mg RE/g. EA extract showed the strongest anti-amylase activity among the tested extracts. Water extract had the most protective activity against plasmid DNA. To indicate cell survival, MIT test was performed against human MCF-7 and MDA-MB-231 breast cancer cells. Half-maximal inhibitory concentration for cells were calculated and used for detection of mechanisms behind the cancer cell death. EA extract showed up-regulation of Bax proapoptotic gene and apoptotic DNA fragmentation activity on highly invasive MDA-MB-231 cells. Beclin-1 and LC3-II autophagy genes were higly expressed after treatment of MCF-7 cells with EA extracts. EA and MeOH extracts inhibited cell migration ability of both cancer cells. Linoleamide, was dominant component in EA extract and caused apoptosis on MDA-MB-231 breast cancer cells via increasing intranuclear Ca2+. The detailed mechanism behind the anticancer properties should be further investigated. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipKastamonu University (Kastamonu University Scientific Projects Research Office) [KUBAP-01/2018-7]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the generous technical and financial support of Kastamonu University (Kastamonu University Scientific Projects Research Office (Project Number KUBAP-01/2018-7).en_US
dc.identifier.citationYerlikaya, S., Baloglu, M. C., Diuzheva, A., Jekő, J., Cziáky, Z., Zengin, G. (2019). Investigation Of Chemical Profile, Biological Properties Of Lotus Corniculatus L. Extracts And Their Apoptotic-Autophagic Effects On Breast Cancer Cells. Journal Of Pharmaceutical And Biomedical Analysis, 174, 286-299.
dc.identifier.doi10.1016/j.jpba.2019.05.068en_US
dc.identifier.endpage299en_US
dc.identifier.issn0731-7085en_US
dc.identifier.issn1873-264Xen_US
dc.identifier.pmid31185340en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage286en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jpba.2019.05.068
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37942
dc.identifier.volume174en_US
dc.identifier.wosWOS:000479328000035en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZengin, Gokhan
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectLotusen_US
dc.subjectExtractsen_US
dc.subjectChemical ingredientsen_US
dc.subjectApoptosisen_US
dc.subjectAutophagyen_US
dc.titleInvestigation of chemical profile, biological properties of Lotus corniculatus L. extracts and their apoptotic-autophagic effects on breast cancer cellsen_US
dc.typeArticleen_US

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