Fully Automated Three-Dimensional Column-Switching SPE-FIA-HPLC System for the Characterization of Lipids by a Single Injection: Part I. Instrumental Design and Chemometric Approach to Assess the Effect of Experimental Settings on the Response of ELSD
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This article presents the first application of fully automated three-dimensional (3D) column-switching SPE-FIA-HPLC system for the characterization of lipids by a single injection. The whole system was designed and set up by modifying Agilent 1200 Series HPLC system in our laboratory. By using this system, a complete separation profile of the oil samples was achieved in a very short time period by using single injections. This approach was applied on vegetable oils which contains a large number of relatively high-class lipid components, such as TG, FFA, sterols, tocopherols, DG, ester and MG. In this part of the study, we focused on the optimization of evaporative light scattering detector (ELSD) by using an experimental design and RSM. Three experimental parameters were chosen as an independent variables which are the flow rate of mobile phase, nebulization temperature and evaporation temperature. A multivariate five level experimental design was used to establish a quadratic model as a functional relationship between the response values and independent variables. The optimal values of parameters were found to be a flow rate of 1.25 mL min(-1), nebulization temperature of 80 degrees C, and evaporation temperature of 40 degrees C. Regression analysis with an R-2 values indicated as a satisfactory correlation between the experimental and predicted values. ANOVA test results were also illustrate that the models can be successfully used to predict the optimum parameters of ELSD. Thus, the proposed system is suitable for a large number of applications including research and development of new quality control and characterization methods for vegetable oils.
Açıklama
Anahtar Kelimeler
Three-dimensional (3D) separation, Column-switching, ELSD, Lipid, Vegetable oil, Chemometric
Kaynak
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
93
Sayı
1
