Fully Automated Three-Dimensional Column-Switching SPE-FIA-HPLC System for the Characterization of Lipids by a Single Injection: Part I. Instrumental Design and Chemometric Approach to Assess the Effect of Experimental Settings on the Response of ELSD

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dc.contributor.authorArslan, Fatma Nur
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T19:24:20Z
dc.date.available2020-03-26T19:24:20Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis article presents the first application of fully automated three-dimensional (3D) column-switching SPE-FIA-HPLC system for the characterization of lipids by a single injection. The whole system was designed and set up by modifying Agilent 1200 Series HPLC system in our laboratory. By using this system, a complete separation profile of the oil samples was achieved in a very short time period by using single injections. This approach was applied on vegetable oils which contains a large number of relatively high-class lipid components, such as TG, FFA, sterols, tocopherols, DG, ester and MG. In this part of the study, we focused on the optimization of evaporative light scattering detector (ELSD) by using an experimental design and RSM. Three experimental parameters were chosen as an independent variables which are the flow rate of mobile phase, nebulization temperature and evaporation temperature. A multivariate five level experimental design was used to establish a quadratic model as a functional relationship between the response values and independent variables. The optimal values of parameters were found to be a flow rate of 1.25 mL min(-1), nebulization temperature of 80 degrees C, and evaporation temperature of 40 degrees C. Regression analysis with an R-2 values indicated as a satisfactory correlation between the experimental and predicted values. ANOVA test results were also illustrate that the models can be successfully used to predict the optimum parameters of ELSD. Thus, the proposed system is suitable for a large number of applications including research and development of new quality control and characterization methods for vegetable oils.en_US
dc.description.sponsorshipProgram for Scientific and Technological Research Projects [1001, 112T373]; Selcuk University Coordinators of Scientific ResearchSelcuk University [12401045, 11201063]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThe present study is a part of the Ph.D. thesis entitled "Integration of retarded and pre-concentrated SPE systems to FIA-HPLC systems for vegetable oil analysis and improvement of its applications". The research was supported by TUBITAK with a 1001-The Support Program for Scientific and Technological Research Projects entitled "Developing a new SPE-FIA-HPLC hyphenated system for three-dimensional chromatographic analysis of vegetable oils with a single injection and their applications" with 112T373 Project number and Selcuk University Coordinators of Scientific Research with 12401045 and 11201063 Project numbers. The authors wish to thank the principal of TUBITAK and Selcuk University S.R.P. Coordination.en_US
dc.identifier.doi10.1007/s11746-015-2750-0en_US
dc.identifier.endpage26en_US
dc.identifier.issn0003-021Xen_US
dc.identifier.issn1558-9331en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage11en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11746-015-2750-0
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33630
dc.identifier.volume93en_US
dc.identifier.wosWOS:000367542300002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofJOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectThree-dimensional (3D) separationen_US
dc.subjectColumn-switchingen_US
dc.subjectELSDen_US
dc.subjectLipiden_US
dc.subjectVegetable oilen_US
dc.subjectChemometricen_US
dc.titleFully Automated Three-Dimensional Column-Switching SPE-FIA-HPLC System for the Characterization of Lipids by a Single Injection: Part I. Instrumental Design and Chemometric Approach to Assess the Effect of Experimental Settings on the Response of ELSDen_US
dc.typeArticleen_US

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