The Effect of Different Hormone Concentrations and Dark Pretreatment on Adventitious Shoot Regeneration in Snake Melon (Cucumis Melo Var. Flexousus)
Yükleniyor...
Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ARS DOCENDI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Explants consisting of the proximal part of cotyledons from 7 day-old seedlings, germinated under dark and light conditions, were cultured on MS media containing different concentrations of BA (0, 0.5, 1.0, 2.0 mg/l) and IAA (0, 0.25, 0.5, 1.0 mg/l). Germination ratio of the seeds under both light and dark conditions was found as 66.8 %, but shoot formation ratio obtained from the seeds germinated under dark condition was higher than the seeds germinated under light condition. The highest frequency of adventitious shoot regeneration (42.8 %) was obtained on MS medium with 1.0 mg/l BA+ 0.25 mg/l IAA from the seeds germinated under dark condition. 1.0 mg/l BA+ 0.5 mg/l IAA (34.8 %), 1.0 mg/l BA (32.8 %), 2.0 mg/l BA+ 0.25 mg/l IAA (12.8 %) and 2.0 mg/l BA+ 0.5 mg/l IAA (7.1 %) resulted in a lower shoot regeneration frequency. In the case of the seeds germinated under light conditions, the maximum frequency of adventitious shoot regeneration from cotyledon explants (2.8 %) was achieved on MS medium with 1.0mg/l BA+ 0.25 mg/l IAA. Hormonal supplements of 2.0 mg/l BA (1.8 %) and 2.0 mg/l BA+ 0.5 mg/l MA (1.4 %) resulted in a much lower shoot regeneration frequency.
Açıklama
Anahtar Kelimeler
Snake Melon, Organogenesis, Cotyledon Explants, Adventitious Shoots
Kaynak
Romanian Biotechnological Letters
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
15
Sayı
4
Künye
Yalçın Mendi, Y., Çömlekçioğlu, N., İpek, M., Kocaman, E., İzgu, T., Tekdal, D., Çürük, P., (2010). The Effect of Different Hormone Concentrations and Dark Pretreatment on Adventitious Shoot Regeneration in Snake Melon (Cucumis Melo Var. Flexousus). Romanian Biotechnological Letters, 15(4), 5392-5395.
