Multiple pharmacological approaches on Fibigia eriocarpa extracts by in vitro and computational assays
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The ethyl acetate, methanolic, and water extracts of Fibigia eriocarpa were assessed for a panoply of bioactivities. Total phenolic and flavonoid content were quantified as well as individual phenolic compounds by HPLC-DAD. The invitro antioxidant and enzyme (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, -amylase, and -glucosidase) inhibitory potential of the extracts were evaluated. In silico molecular docking was used to investigate possible interaction between dominant compounds and selected enzymes. Vanillin (303g/g extract), apigenin (270g/g extract), and kaempferol (180g/g extract) were the main compounds in the ethyl acetate extract, while the methanolic extract was characterized by the presence of vanillin, rutin, and apigenin (616, 616 and 252g/g extract, respectively). (+)-catechin (1422g/g extract) was the main compound in the water extracts. The ethyl acetate extract was found to be a superior source of antioxidant compounds and enzyme inhibitors against above-mentioned enzymes. Docking studies revealed that p-hydroxybenzoic and (+)-catechin have the best scores for tyrosinase, while kaempferol and apigenin showed the best binding pose for -glucosidase, AChE, and BChE. Results amassed herein are the first report on the phytochemical and biological attributes of F.eriocarpa, which tend to validate the pharmacological uses of this plant as an alternative medicine.
Açıklama
Anahtar Kelimeler
antioxidant, bioactive compounds, Fibigia, molecular docking, natural enzyme inhibitors
Kaynak
FUNDAMENTAL & CLINICAL PHARMACOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
32
Sayı
4
