Investigation of developmental toxicity and teratogenicity of macrolide antibiotics in cultured rat embryos

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dc.contributor.authorKarabulut, A. K.
dc.contributor.authorUysal, I. I.
dc.contributor.authorAcar, H.
dc.contributor.authorFazliogullari, Z.
dc.date.accessioned2020-03-26T17:27:05Z
dc.date.available2020-03-26T17:27:05Z
dc.date.issued2008
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractMacrolides are considered to be one of the safest anti-infective groups in clinical use, with severe adverse reactions being rare. However, there are limited data about their embryotoxicity and teratogenicity. We aimed to investigate and compare the effects of these agents on embryonic growth and development. Rat embryos were cultured in vitro for 48 h in rat serum. Whole rat serum was used as a culture medium for the control group while different concentrations of spiramycin and azithromycin (1.25-6.25 mu g/ml), and clarithromycin (2.5-30 mu g/ml) were added to rat serum for the experimental groups. Dose-dependent effects of macrolides on embryonic developmental parameters were compared using morphological methods. Embryos were evaluated for the presence of any malformations. After morphological examination of the embryos, total DNA was extracted from the cells using standard procedures to determine fragmentation of nuclear DNA of embryonic cells. When compared with the control embryos, the macrolides significantly decreased all growth and developmental parameters dose dependently. While clarithromycin was found to cause more developmental toxicity than spiramycin and azithromycin, azitromycin was determined to have more teratogenicity potential. Compared with controls, there was no difference regarding the fragmentation of nuclear DNA of all the agents used. According to these results, when the toxic and teratogenic potential of the used agents compared, because of the lower toxic and teratogenic effects observed with spiramycin, this agent may be preferred for parturients.en_US
dc.description.sponsorshipSelcuk University Research FoundationSelcuk Universityen_US
dc.description.sponsorshipThis study was supported by the Selcuk University Research Foundation.en_US
dc.identifier.doi10.1111/j.1439-0264.2008.00861.xen_US
dc.identifier.endpage375en_US
dc.identifier.issn0340-2096en_US
dc.identifier.issn1439-0264en_US
dc.identifier.issue5en_US
dc.identifier.pmid18537945en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage369en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1439-0264.2008.00861.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/22468
dc.identifier.volume37en_US
dc.identifier.wosWOS:000258969500009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofANATOMIA HISTOLOGIA EMBRYOLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleInvestigation of developmental toxicity and teratogenicity of macrolide antibiotics in cultured rat embryosen_US
dc.typeArticleen_US

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