Visualization of red-ox proteins on the gold surface using enzymatic polypyrrole formation
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER WIEN
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We describe a new method for the visualization of the activity of red-ox proteins on a gold interface. Glucose oxidase was selected as a model system. Surfaces were modified by adhesion of glucose oxidase on (a) electrochemically cleaned gold; (b) gold films modified with gold nanoparticles, (c) a gold surface modified with self-assembled monolayer, and (d) covalent immobilization of protein on the gold surface modified with a self-assembled monolayer. The simple optical method for the visualization of enzyme on the surfaces is based on the enzymatic formation of polypyrrole. The activity of the enzyme was quantified via enzymatic formation of polypyrrole, which was detected and investigated by quartz microbalance and amperometric techniques. The experimental data suggest that the enzymatic formation of the polymer may serve as a method to indicate the adhesion of active redox enzyme on such surfaces.
Açıklama
Anahtar Kelimeler
Glucose oxidase, Polypyrrole, Enzymatic synthesis, Self-assembled monolayer, Gold surface
Kaynak
MICROCHIMICA ACTA
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
175
Sayı
01.02.2020
