Visualization of red-ox proteins on the gold surface using enzymatic polypyrrole formation

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dc.contributor.authorRamanaviciene, Almira
dc.contributor.authorKausaite-Minkstimiene, Asta
dc.contributor.authorOztekin, Yasemin
dc.contributor.authorCarac, Geta
dc.contributor.authorVoronovic, Jaroslav
dc.contributor.authorGerman, Natalija
dc.contributor.authorRamanavicius, Arunas
dc.date.accessioned2020-03-26T18:17:32Z
dc.date.available2020-03-26T18:17:32Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractWe describe a new method for the visualization of the activity of red-ox proteins on a gold interface. Glucose oxidase was selected as a model system. Surfaces were modified by adhesion of glucose oxidase on (a) electrochemically cleaned gold; (b) gold films modified with gold nanoparticles, (c) a gold surface modified with self-assembled monolayer, and (d) covalent immobilization of protein on the gold surface modified with a self-assembled monolayer. The simple optical method for the visualization of enzyme on the surfaces is based on the enzymatic formation of polypyrrole. The activity of the enzyme was quantified via enzymatic formation of polypyrrole, which was detected and investigated by quartz microbalance and amperometric techniques. The experimental data suggest that the enzymatic formation of the polymer may serve as a method to indicate the adhesion of active redox enzyme on such surfaces.en_US
dc.description.sponsorshipEuropean UnionEuropean Union (EU) [SF-PD-2009-08-17-0151]en_US
dc.description.sponsorshipThe authors would like to thank European Union Structural Funds project "Postdoctoral Fellowship Implementation in Lithuania", SF-PD-2009-08-17-0151 for the financial support.en_US
dc.identifier.doi10.1007/s00604-011-0645-9en_US
dc.identifier.endpage86en_US
dc.identifier.issn0026-3672en_US
dc.identifier.issue01.02.2020en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage79en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00604-011-0645-9
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27043
dc.identifier.volume175en_US
dc.identifier.wosWOS:000295169300009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGER WIENen_US
dc.relation.ispartofMICROCHIMICA ACTAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGlucose oxidaseen_US
dc.subjectPolypyrroleen_US
dc.subjectEnzymatic synthesisen_US
dc.subjectSelf-assembled monolayeren_US
dc.subjectGold surfaceen_US
dc.titleVisualization of red-ox proteins on the gold surface using enzymatic polypyrrole formationen_US
dc.typeArticleen_US

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