Metabolomic markers of fertility in bull seminal plasma

Basit Öğe Kaydı
dc.contributor.authorVelho, Ana Luiza Cazaux
dc.contributor.authorMenezes, Erika
dc.contributor.authorThu Dinh
dc.contributor.authorKaya, Abdullah
dc.contributor.authorTopper, Einko
dc.contributor.authorMoura, Arlindo Alencar
dc.contributor.authorMemili, Erdogan
dc.date.accessioned2020-03-26T19:54:39Z
dc.date.available2020-03-26T19:54:39Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractMetabolites play essential roles in biological systems, but detailed identities and significance of the seminal plasma metabolome related to bull fertility are still unknown. The objectives of this study were to determine the comprehensive metabolome of seminal plasma from Holstein bulls and to ascertain the potential of metabolites as biomarkers of bull fertility. The seminal plasma metabolome from 16 Holstein bulls with two fertility rates were determined by gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analyses of the data were performed, and the pathways associated with the seminal plasma metabolome were identified using bioinformatics approaches. Sixty-three metabolites were identified in the seminal plasma of all bulls. Fructose was the most abundant metabolite in the seminal fluid, followed for citric acid, lactic acid, urea and phosphoric acid. Androstenedione, 4-ketoglucose, D-xylofuranose, 2-oxoglutaric acid and erythronic acid represented the least predominant metabolites. Partial-Least Squares Discriminant Analysis (PLSDA) revealed a distinct separation between high and low fertility bulls. The metabolites with the greatest Variable Importance in Projection score (VIP > 2) were 2-oxoglutaric acid and fructose. Heat-map analysis, based on VIP score, and univariate analysis indicated that 2-oxoglutaric acid was less (P = 0.02); whereas fructose was greater (P = 0.02) in high fertility than in low fertility bulls. The current study is the first to describe the metabolome of bull seminal plasma using GC-MS and presented metabolites such as 2-oxoglutaric acid and fructose as potential biomarkers of bull fertility.en_US
dc.description.sponsorshipAgriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture (NIFA) [2017-67016-26507]; Mississippi Agricultural Experiment Station; Alta Genetics Inc.; Hatch project from the USDA NIFA [1005778]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of BrazilNational Council for Scientific and Technological Development (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of BrazilCAPESen_US
dc.description.sponsorshipThis project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2017-67016-26507 from the USDA National Institute of Food and Agriculture (NIFA). Partial funding was also provided by Mississippi Agricultural Experiment Station, Alta Genetics Inc., Hatch project under accession number 1005778 from the USDA NIFA, and by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of Brazil. A. Velho and E. Menezes were funded by competitive fellowships from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of Brazil.en_US
dc.identifier.doi10.1371/journal.pone.0195279en_US
dc.identifier.issn1932-6203en_US
dc.identifier.issue4en_US
dc.identifier.pmid29634739en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1371/journal.pone.0195279
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36773
dc.identifier.volume13en_US
dc.identifier.wosWOS:000429522200027en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.relation.ispartofPLOS ONEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleMetabolomic markers of fertility in bull seminal plasmaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu