Immobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfaces

Basit Öğe Kaydı
dc.contributor.authorGübbük, İlkay Hilal
dc.contributor.authorÖzmen, Mustafa
dc.contributor.authorMaltaş, Esra
dc.date.accessioned2020-03-26T18:30:42Z
dc.date.available2020-03-26T18:30:42Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractHemoglobin was covalently immobilized onto modified sporopollenin surface with different functional groups by chemical reactions to enhance binding ability of protein. In this study, the influence of various silane linker molecules on the capacity of protein binding was studied. For this purpose, activated sporopollenin was modified by 3-aminopropyltriethoxysilane (APTS), 3-chloropropyltrimethoxysilane (CPTS) and (3-glycidyloxypropyl)trimethoxysilane (GPTS). Hemoglobin (Hb) was immobilized on modified sporopollenin surfaces in phosphate buffer saline solution (PBS, pH 7.4) at 4 degrees C. Results showed that GPTS modified sporopollenin surfaces resulted in the highest binding capacity for Hb. Micro porosity of samples was observed through scanning electron microscopy (SEM) and thermal behavior of the samples were studied with thermogravimetric analysis (TGA) within a temperature range: 25-900 degrees C. TGA studies demonstrated the advantages of silane modification for high temperature applications and illustrated differences of the structures due to the different tail groups.en_US
dc.description.sponsorshipResearch Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipWe would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work.en_US
dc.identifier.citationGübbük, I. H., Özmen, M., Maltaş, E., (2012). Immobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfaces. International Journal of Biological Macromolecules, (50), 1346-1352. Doi: 10.1016/j.ijbiomac.2012.04.009
dc.identifier.doi10.1016/j.ijbiomac.2012.04.009en_US
dc.identifier.endpage1352en_US
dc.identifier.issn0141-8130en_US
dc.identifier.pmid22537474en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1346en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2012.04.009
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28124
dc.identifier.volume50en_US
dc.identifier.wosWOS:000304848300025en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorMaltaş, Esra
dc.institutionauthorÖzmen, Mustafa
dc.institutionauthorGübbük, İlkay Hilal
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSporopolleninen_US
dc.subjectHemoglobinen_US
dc.subjectModificationen_US
dc.subjectSilaneen_US
dc.subjectHorowitz-Metzger methoden_US
dc.titleImmobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfacesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
28124.pdf
Boyut:
1.03 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Kimya/Makale Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu