Immobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfaces
dc.contributor.author | Gübbük, İlkay Hilal | |
dc.contributor.author | Özmen, Mustafa | |
dc.contributor.author | Maltaş, Esra | |
dc.date.accessioned | 2020-03-26T18:30:42Z | |
dc.date.available | 2020-03-26T18:30:42Z | |
dc.date.issued | 2012 | |
dc.department | Selçuk Üniversitesi | en_US |
dc.description.abstract | Hemoglobin was covalently immobilized onto modified sporopollenin surface with different functional groups by chemical reactions to enhance binding ability of protein. In this study, the influence of various silane linker molecules on the capacity of protein binding was studied. For this purpose, activated sporopollenin was modified by 3-aminopropyltriethoxysilane (APTS), 3-chloropropyltrimethoxysilane (CPTS) and (3-glycidyloxypropyl)trimethoxysilane (GPTS). Hemoglobin (Hb) was immobilized on modified sporopollenin surfaces in phosphate buffer saline solution (PBS, pH 7.4) at 4 degrees C. Results showed that GPTS modified sporopollenin surfaces resulted in the highest binding capacity for Hb. Micro porosity of samples was observed through scanning electron microscopy (SEM) and thermal behavior of the samples were studied with thermogravimetric analysis (TGA) within a temperature range: 25-900 degrees C. TGA studies demonstrated the advantages of silane modification for high temperature applications and illustrated differences of the structures due to the different tail groups. | en_US |
dc.description.sponsorship | Research Foundation of Selcuk University (BAP)Selcuk University | en_US |
dc.description.sponsorship | We would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work. | en_US |
dc.identifier.citation | Gübbük, I. H., Özmen, M., Maltaş, E., (2012). Immobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfaces. International Journal of Biological Macromolecules, (50), 1346-1352. Doi: 10.1016/j.ijbiomac.2012.04.009 | |
dc.identifier.doi | 10.1016/j.ijbiomac.2012.04.009 | en_US |
dc.identifier.endpage | 1352 | en_US |
dc.identifier.issn | 0141-8130 | en_US |
dc.identifier.pmid | 22537474 | en_US |
dc.identifier.scopusquality | Q1 | en_US |
dc.identifier.startpage | 1346 | en_US |
dc.identifier.uri | https://dx.doi.org/10.1016/j.ijbiomac.2012.04.009 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12395/28124 | |
dc.identifier.volume | 50 | en_US |
dc.identifier.wos | WOS:000304848300025 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.institutionauthor | Maltaş, Esra | |
dc.institutionauthor | Özmen, Mustafa | |
dc.institutionauthor | Gübbük, İlkay Hilal | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science Bv | en_US |
dc.relation.ispartof | International Journal of Biological Macromolecules | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.selcuk | 20240510_oaig | en_US |
dc.subject | Sporopollenin | en_US |
dc.subject | Hemoglobin | en_US |
dc.subject | Modification | en_US |
dc.subject | Silane | en_US |
dc.subject | Horowitz-Metzger method | en_US |
dc.title | Immobilization and Characterization of Hemoglobin on Modified Sporopollenin Surfaces | en_US |
dc.type | Article | en_US |
Dosyalar
Orijinal paket
1 - 1 / 1
Yükleniyor...
- İsim:
- 28124.pdf
- Boyut:
- 1.03 MB
- Biçim:
- Adobe Portable Document Format
- Açıklama:
- Makale Dosyası