New water soluble Hg2+ selective fluorescent calix[4]arenes: Synthesis and application in living cells imaging
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PERGAMON-ELSEVIER SCIENCE LTD
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study demonstrates the synthesis of water-soluble fluorescent calix[4]arenes (6 and 7) and its application in living cell imaging for Hg2+ detection at a low level. The synthesized fluorescent ligands 6 and 7 were characterized by H-1 NMR technique. The fluorescent study showed both water soluble ligands were Hg2+ selective and follow photo-induced electron transfer (PET) process. From the fluorimeter titration experiment detection limit was calculated as 1.14 x 10(-5) and 3.42 x 10(-5) for ligand 6 and 7, respectively. From the Benesi-Hildebrand plot binding constant values were evaluated as 666.7 and 733.3 M-1 for 6 and 7, respectively. The interactions between ligands 6 and 7 and Hg2+ were also demonstrated in living cells, SW-620, using Fluorescent Cell Imager. While ligands 6 and 7 alone show fluorescent properties, they loss their action with the presence of Hg2+ in SW-620 cells. (C) 2016 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
p-Sulphonatocalix[4]arene, Dansyl, Fluorescence, Living cell imaging
Kaynak
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
171
