Regulation of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases by basic fibroblast growth factor and dexamethasone in periodontal ligament cells

Basit Öğe Kaydı
dc.contributor.authorHakki, S. S.
dc.contributor.authorHakki, E. E.
dc.contributor.authorNohutcu, R. M.
dc.date.accessioned2020-03-26T17:39:57Z
dc.date.available2020-03-26T17:39:57Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground and Objectives: In this study, we investigated the effect of basic fibroblast growth factor (bFGF) and dexamethasone (Dex) on mRNA expressions of collagen (COL) type I, III and X, matrix metalloproteinases (MMP)-1, -2, -3 and -9 and tissue inhibitors of metalloproteinases (TIMP)-1 and -2, and also on mineralization and morphology of periodontal ligament (PDL) cells. Material and Methods: Periodontal ligament cells were obtained from premolar teeth extracted for orthodontic reasons. Periodontal ligament cells were cultured with Dulbecco's modified Eagle's medium containing: (1) 5% fetal bovine serum (FBS); (2) 5% FBS + ascorbic acid (AA, 50 mu g/mL); (3) 5% FBS + Dex (10(-7) M) + AA; (4) 5% FBS + bFGF (10 ng/mL) + AA; or (5) 5% FBS + Dex (10(-7) M) + bFGF + AA. Cells within each group were evaluated for gene expression profile using semi-quantitative reverse transcriptase-polymerase chain reaction for COL I, III and X, MMP-1, -2, -3 and -9 and TIMP-1 and -2 on days 14 and 21 and for biomineralization by von Kossa stain in vitro on day 21. Images of PDL cells were examined using a phase contrast microscope. Results: Basic fibroblast growth factor stimulated MMP-1, MMP-3 and MMP-9 mRNA expressions and inhibited TIMP-2 mRNA expression. Treatment of cells with Dex + bFGF led to downregulation of MMP-1, MMP-3 and MMP-9 transcripts. Whilst AA alone and Dex alone induced biomineralization of PDL cells, bFGF blocked the mineralization activity of the cells. In the Dex + bFGF group, more mineral nodules were noted when compared to AA alone and Dex alone groups. Conclusion: The addition of Dex to culture reversed bFGF-mediated inhibition of mineralization. Use of combined bFGF and Dex to regulate PDL cell function may be a good therapeutic option to obtain periodontal regeneration.en_US
dc.description.sponsorshipTurkish Scientific Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG/2648]; Selcuk University Research FoundationSelcuk University [BAP 2002/105, 2002/190]en_US
dc.description.sponsorshipThis study was performed at The Research Laboratory of Faculty of Dentistry, Selcuk University and was supported by Turkish Scientific Research Council (SBAG/2648) and Selcuk University Research Foundation (BAP 2002/105 and 2002/190), Turkey.en_US
dc.identifier.doi10.1111/j.1600-0765.2008.01192.xen_US
dc.identifier.endpage802en_US
dc.identifier.issn0022-3484en_US
dc.identifier.issn1600-0765en_US
dc.identifier.issue6en_US
dc.identifier.pmid19602122en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage794en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1600-0765.2008.01192.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23818
dc.identifier.volume44en_US
dc.identifier.wosWOS:000271062400014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofJOURNAL OF PERIODONTAL RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectperiodontal ligament cellen_US
dc.subjectperiodontal regenerationen_US
dc.subjectbasic fibroblast growth factoren_US
dc.subjectmatrix metalloproteinaseen_US
dc.subjecttissue inhibitor of metalloproteinasesen_US
dc.titleRegulation of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases by basic fibroblast growth factor and dexamethasone in periodontal ligament cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu