Effect of avulsion storage media on periodontal ligament fibroblast differentiation

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dc.contributor.authorBag, Irem
dc.contributor.authorYildirim, Sibel
dc.date.accessioned2020-03-26T19:35:03Z
dc.date.available2020-03-26T19:35:03Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground/AimAn avulsed tooth must be stored in a solution which maintains periodontal ligament (PDL) cell viability. The aim of this study was to compare the effects of Hank's balanced salt solution (HBSS) and milk on the differentiation of PDL fibroblasts. Materials and methodsEighteen extracted third molars, (n=3 for each group), were immersed in HBSS, milk, and Dulbecco's modified Eagle medium-Ham's F12 (DMEM-F12) at 4 degrees C for 30- to 60 minutes or 12hours. The growth dynamics of PDL fibroblasts were evaluated with cell proliferation graphics and population doubling time (PDT) values. Runt-related transcription factor 2 (RUNX2), receptor activator of nuclear factor kappa-B ligand (RANKL), and collagen type XII (COL12) expression were used to evaluate the differentiation of PDL fibroblasts. ResultsThe percentage of cell numbers and PDT values of groups were statistically insignificant. In the HBSS groups, RUNX2 expression increased showing a direction to osteogenic differentiation of PDL fibroblasts. In the DMEM-F12 groups, RANKL expression increased, indicating there was a tendency for osteoclastogenic differentiation. In the milk groups, RUNX2 expression decreased while other markers were stable showing PDL fibroblasts could protect fibroblast identity. ConclusionsIn terms of protecting fibroblast identity and resistance to differentiation, milk was more effective than HBSS.en_US
dc.identifier.doi10.1111/edt.12356en_US
dc.identifier.endpage464en_US
dc.identifier.issn1600-4469en_US
dc.identifier.issn1600-9657en_US
dc.identifier.issue6en_US
dc.identifier.pmid28715096en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage458en_US
dc.identifier.urihttps://dx.doi.org/10.1111/edt.12356
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34994
dc.identifier.volume33en_US
dc.identifier.wosWOS:000416016300007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofDENTAL TRAUMATOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcell differentiationen_US
dc.subjectfibroblasten_US
dc.subjectperiodontal ligamenten_US
dc.subjectstorage mediaen_US
dc.subjecttooth avulsionen_US
dc.titleEffect of avulsion storage media on periodontal ligament fibroblast differentiationen_US
dc.typeArticleen_US

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