The effect of maras powder on DNA methylation and micronucleus formation in human buccal tissue

dc.contributor.authorSaatci, Cetin
dc.contributor.authorOzkul, Yusuf
dc.contributor.authorTahiri, Serpil
dc.contributor.authorCaglayan, Ahmet Okay
dc.contributor.authorTurhan, Ahmet Bulent
dc.contributor.authorDundar, Munis
dc.date.accessioned2020-03-26T17:28:03Z
dc.date.available2020-03-26T17:28:03Z
dc.date.issued2008
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe plant powder "maras powder" (MP) has been used widely instead of cigarettes in the southeastern region of Turkey. The aim of this study was to assess the impacts of MP and cigarette smoking on the methylation and micronuclei (MN) formation in buccal cells of humans with a comparison to blood lymphocytes. DNA samples from 80 subjects (40 MP users, 20 tobacco smokers, 20 healthy volunteers) were analyzed for their genomic methylation status using Hpa II and Msp I digestions followed by a simple gel electrophoresis and ethidium bromide staining. A densitometric method was developed to measure the methylation in genomic DNA samples and the results were evaluated using a software program designed for this purpose. Buccal epithelial cells were collected from the same groups and examined for MN formation. The results indicated that a general genomic hypomethylation was present in almost all of the samples that were obtained from MP users and tobacco smokers. This hypomethylation was significant in MP users compared to smokers and healthy volunteers. The percentage of cells containing MN was 1.93 in MP users, 0.95 in healthy volunteers, and 1.82 in smokers. The MN frequency was significantly higher in MP users and smokers than in healthy volunteers. There was no statistical difference between smokers and MP users. Evidence indicates that MP usage induces DNA hypomethylation and increase frequency of MN formation.en_US
dc.identifier.doi10.1080/15287390701801661en_US
dc.identifier.endpage404en_US
dc.identifier.issn1528-7394en_US
dc.identifier.issue6en_US
dc.identifier.pmid18246499en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage396en_US
dc.identifier.urihttps://dx.doi.org/10.1080/15287390701801661
dc.identifier.urihttps://hdl.handle.net/20.500.12395/22705
dc.identifier.volume71en_US
dc.identifier.wosWOS:000253154000006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofJOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleThe effect of maras powder on DNA methylation and micronucleus formation in human buccal tissueen_US
dc.typeArticleen_US

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