Comparison of five methods for isolation of DNA from Mycoplasma cynos

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dc.contributor.authorSakmanoglu, Ash
dc.contributor.authorSayin, Zafer
dc.contributor.authorUcan, Uckun Sait
dc.contributor.authorPinarkara, Yasemin
dc.contributor.authorUslu, Ali
dc.contributor.authorErganis, Osman
dc.date.accessioned2020-03-26T19:34:28Z
dc.date.available2020-03-26T19:34:28Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractExtraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute (TM) gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute (TM) gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods. The results were evaluated based on the purity and amount of DNA obtained from one Mycoplasma colony. Eight strains of Mycoplasma cynos isolated from the broncho-alveolar lavage fluid of dogs were used. The GenElute (TM) gel extraction protocol was the most efficient among all the methods tested in this study as it yielded the highest amount and the purest quality of DNA (199.3 +/- 0.744 ng/mu 1) from a single colony. Among the methods tested, the GenElute (TM) gel extraction method is the most rapid, sensitive, and simple method for DNA extraction from Mycoplasma. This procedure may also prove useful for extracting DNA from other Mycoplasma species.en_US
dc.description.sponsorshipCoordinatorship for Scientific Research Projects of Selcuk University [Konya, Turkey] [SUBAPK 17401041]en_US
dc.description.sponsorshipThis study was part of research project supported by the Coordinatorship for Scientific Research Projects of Selcuk University [SUBAPK 17401041, Konya, Turkey].en_US
dc.identifier.doi10.1016/j.mimet.2017.07.003en_US
dc.identifier.endpage73en_US
dc.identifier.issn0167-7012en_US
dc.identifier.issn1872-8359en_US
dc.identifier.pmid28711528en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.mimet.2017.07.003
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34901
dc.identifier.volume140en_US
dc.identifier.wosWOS:000407982100012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofJOURNAL OF MICROBIOLOGICAL METHODSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMycoplasma cynosen_US
dc.subjectNucleic acid extractionen_US
dc.subjectPCRen_US
dc.titleComparison of five methods for isolation of DNA from Mycoplasma cynosen_US
dc.typeArticleen_US

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