Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

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dc.contributor.authorLocatelli, Marcello
dc.contributor.authorMacchione, Nicola
dc.contributor.authorFerrante, Claudio
dc.contributor.authorChiavaroli, Annalisa
dc.contributor.authorRecinella, Lucia
dc.contributor.authorCarradori, Simone
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T19:54:06Z
dc.date.available2020-03-26T19:54:06Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractProstatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents. The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation. Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis. The aim of the present work was to evaluate the protective effects of Graminex pollen (Graminex(TM), Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo. In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E-2 (PGE(2)), nuclear factor kappa-light-chain-enhancer of activated B cells (NFB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli. We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS). We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFB mRNA, and PGE(2) levels, in rat prostate specimens. According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.en_US
dc.description.sponsorshipItalian MIURMinistero dell' Istruzione, dell' Universita e della Ricerca (MIUR) [FAR 2016]en_US
dc.description.sponsorshipThis work was supported by local grants from Italian MIUR (FAR 2016).en_US
dc.identifier.doi10.3390/molecules23051145en_US
dc.identifier.issn1420-3049en_US
dc.identifier.issue5en_US
dc.identifier.pmid29751604en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.3390/molecules23051145
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36662
dc.identifier.volume23en_US
dc.identifier.wosWOS:000435204000160en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectpollenen_US
dc.subjectPGE(2)en_US
dc.subjectNFB mRNAen_US
dc.subjectphenolic patternen_US
dc.subjectcolorimetric analysisen_US
dc.subjectinflammationen_US
dc.titleGraminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPSen_US
dc.typeArticleen_US

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