Ionizing Radiation Induces Cytokines, MMP-1, TIMP-1 and Supresses Type I Collagen mRNA Expressions in Human Gingival Fibroblasts

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dc.contributor.authorYavas, Guler
dc.contributor.authorYavas, Cagdas
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorAta, Ozlem
dc.contributor.authorHakki, Sema
dc.date.accessioned2020-03-26T18:51:17Z
dc.date.available2020-03-26T18:51:17Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractWe aimed to evaluate the effects of ionizing radiation on the proliferation of gingival fibroblasts and the expressions of proinflammatory cytokines and matrix metalloproteinase-1 (MMP-1), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and type I collagen (type I Col) mRNA transcripts. Gingival fibroblasts were treated with radiation doses as follows; 0.5 Gy, 1 Gy, 2 Gy, 4 Gy, 6 Gy, and 8 Gy. Expression of interleukin (IL)-1 beta, IL-6, IL-8 and, MMP-1, TIMP-1 and of type I Col mRNA transcripts in human gingival fibroblasts was determined by quantitative polymerase chain reaction (PCR) analysis. Morphology of gingival fibroblasts was evaluated using inverted microscope. Ionizing radiation decreased cell proliferation (p< 0.05) compared to the control group. Expressions of IL-1 beta, IL-6 and IL-8 were stimulated at the highest dosage of radiation (p< 0.001). In parallel to proinflammtory cytokines, MMP-1 and TIMP-1 mRNA expressions were elevated in response to higher dosage of radiation (p< 0.001). Radiation suppressed type I Col mRNA expression in response to all doses at 24 hrs (p< 0.001). In addition to basal epithelial cells of the oral mucosa, gingival fibroblasts have an important role in the pathogenesis of oral mucositis. Results of this study may help to clarify the role of gingival fibroblasts in radiation induced oral mucositis.en_US
dc.identifier.doi10.4999/uhod.14371en_US
dc.identifier.endpage156en_US
dc.identifier.issn1306-133Xen_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage149en_US
dc.identifier.urihttps://dx.doi.org/10.4999/uhod.14371
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30953
dc.identifier.volume24en_US
dc.identifier.wosWOS:000342543200001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherAKAD DOKTORLAR YAYINEVIen_US
dc.relation.ispartofUHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGingival fibroblasten_US
dc.subjectIonizing radiationen_US
dc.subjectOral mucositisen_US
dc.subjectProinflammatory cytokinesen_US
dc.titleIonizing Radiation Induces Cytokines, MMP-1, TIMP-1 and Supresses Type I Collagen mRNA Expressions in Human Gingival Fibroblastsen_US
dc.typeArticleen_US

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