Sphaerophysa kotschyana, an endemic species from Central Anatolia: antioxidant system responses under salt stress

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dc.contributor.authorYildiztugay, Evren
dc.contributor.authorOzfidan-Konakci, Ceyda
dc.contributor.authorKucukoduk, Mustafa
dc.date.accessioned2020-03-26T18:43:29Z
dc.date.available2020-03-26T18:43:29Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSphaerophysa kotschyana is a Turkish endemic and endangered plant that grows near Salt Lake, in Konya, Turkey. However, little is known about the ability of this plant to generate/remove reactive oxygen species (ROS) or its adaptive biochemical responses to saline environments. After exposure of S. kotschyana to 0, 150, and 300 mM NaCl for 7 and 14 days, we investigated (1) the activities and isozyme compositions of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), and glutathione reductase (GR); (2) the oxidative stress parameters NADPH oxidase (NOX) activity, lipid peroxidation (MDA), total ascorbate (tAsA) content, and total glutathione content (tGlut); and (3) ROS levels for superoxide anion radical (O (2) (center dot-) ), hydrogen peroxide (H2O2), hydroxyl radicals (OH center dot), and histochemical staining of O (2) (center dot-) and H2O2. H2O2 content increased after 14 days of salt stress, which was consistent with the results from histochemical staining and NOX activity measurements. In contrast, oxidative stress induced by 150 mM NaCl was more efficiently prevented, as indicated by low malondialdehyde (MDA) levels and especially at 7 days, by increased levels of SOD, POX, APX, and GR. However, at 300 mM NaCl, decreased levels of protective enzymes such as SOD, CAT, POX, and GR, particularly with long-term stress (14 days), resulted in limited ROS scavenging activity and increased MDA levels. Moreover, at 300 mM NaCl, the high H2O2 content caused oxidative damage rather than inducing protective responses against H2O2. These results suggest that S. kotschyana is potentially tolerant to salt-induced damage only at low salt concentrations.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinating OfficeSelcuk University [11401069]en_US
dc.description.sponsorshipFinancial support for this work was provided by Selcuk University Scientific Research Projects Coordinating Office (Project number: 11401069). We gratefully thank to Dr. Mirza Hasanuzzaman Assist. Prof., department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh, for his recommendation on performing on analysis of tAsA and tGlut contents and language correction. We also would like to thank to Dr. Mehmet Hamurcu Assist. Prof., for his technical assistance on ICP-OES analyses.en_US
dc.identifier.doi10.1007/s10265-013-0573-3en_US
dc.identifier.endpage742en_US
dc.identifier.issn0918-9440en_US
dc.identifier.issue5en_US
dc.identifier.pmid23761064en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage729en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10265-013-0573-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29837
dc.identifier.volume126en_US
dc.identifier.wosWOS:000323502000016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGER JAPAN KKen_US
dc.relation.ispartofJOURNAL OF PLANT RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntioxidant enzymesen_US
dc.subjectMalondialdehydeen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectSalt stressen_US
dc.subjectSphaerophysa kotschyanaen_US
dc.titleSphaerophysa kotschyana, an endemic species from Central Anatolia: antioxidant system responses under salt stressen_US
dc.typeArticleen_US

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