Novel humic acid-bonded magnetite nanoparticles for protein immobilization

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dc.contributor.authorBayrakci, Mevlut
dc.contributor.authorGezici, Orhan
dc.contributor.authorBas, San Zeki
dc.contributor.authorOzmen, Mustafa
dc.contributor.authorMaltas, Esra
dc.date.accessioned2020-03-26T18:51:47Z
dc.date.available2020-03-26T18:51:47Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe present paper is the first report that introduces (i) a useful methodology for chemical immobilization of humic acid (HA) to aminopropyltriethoxysilane-functionalized magnetite iron oxide nanoparticles (APS-MNPs) and (ii) human serum albumin (HSA) binding to the obtained material (HA-APS-MNPs). The newly prepared magnetite nanoparticle was characterized by using Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and elemental analysis. Results indicated that surface modification of the bare magnetite nanoparticles (MNPs) with aminopropyltriethoxysilane (APS) and HA was successfully performed. The protein binding studies that were evaluated in batch mode exhibited that HA-APS-MNPs could be efficiently used as a substrate for the binding of HSA from aqueous solutions. Usually, recovery values higher than 90% were found to be feasible by HA-APS-MNPs, while that value was around 2% and 70% in the cases of MNPs and APS-MNPs, respectively. Hence, the capacity of MNPs was found to be significantly improved by immobilization of HA. Furthermore, thermal degradation of HA-APS-MNPs and HSA bonded HA-APS-MNPs was evaluated in terms of the Horowitz-Metzger equation in order to determine kinetic parameters for thermal decomposition. Activation energies calculated for HA-APS-MNPs (20.74 kJ mol(-1)) and HSA bonded HA-APS-MNPs (33.42 kJ mol(-1)) implied chemical immobilization of HA to APS-MNPs, and tight interactions between HA and HA-APS-MNPs. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK through the 2219-International Post-Doctoral Research Fellowship ProgrammeTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThe authors wish to thank Nigde University and Selcuk University for the facilities provided. One of the authors (O. Gezici) wishes to thank TUBITAK for the scholarship provided through the 2219-International Post-Doctoral Research Fellowship Programme, and ETH Zurich for library facilities.en_US
dc.identifier.doi10.1016/j.msec.2014.05.066en_US
dc.identifier.endpage552en_US
dc.identifier.issn0928-4931en_US
dc.identifier.issn1873-0191en_US
dc.identifier.pmid25063152en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage546en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2014.05.066
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31028
dc.identifier.volume42en_US
dc.identifier.wosWOS:000340687400069en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHumic substancesen_US
dc.subjectHuman serum albumin (HSA)en_US
dc.subjectMagnetite nanoparticleen_US
dc.subjectProtein bindingen_US
dc.subjectHorowitz-Metzger methoden_US
dc.titleNovel humic acid-bonded magnetite nanoparticles for protein immobilizationen_US
dc.typeArticleen_US

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