RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey

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dc.contributor.authorVural, H. C.
dc.contributor.authorMaltas, E.
dc.date.accessioned2020-03-26T18:31:15Z
dc.date.available2020-03-26T18:31:15Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractRT-qPCR was used to analyze the vitamin D receptor (VDR) gene TaqI polymorphism in 100 Turkish patients with type 2 diabetes mellitus (T2DM) and hypertension compared with 100 healthy subjects, to determine whether VDR could be considered as one of the susceptibility genes for T2DM and hypertension. Genotyping was done with PCR, followed by melting curve analysis with specific fluorescent hybridization probes. The results showed that distributions for TT, Tt and tt genotypes were 51, 46 and 3% in the patient group, and 35, 49 and 16% in the control group, respectively. The frequency of the T allele in patients was also significantly higher than that in controls. Based on the results, the relationship between the VDR gene TaqI polymorphism and T2DM patients in the Turkish population was compared. In terms of the genotype distributions and allele frequencies of the VDR gene TaqI polymorphism, there was no statistically significant difference (P > 0.05) between the T2DM and hypertension patients and controls. Application of RT-qPCR method enabled us to assess the prevalence of the VDR gene TaqI polymorphism and its association with type 2 diabetes and hypertension.en_US
dc.description.sponsorshipUniversity of Selcuk, Medical Faculty, Meram, Konya, TurkeySelcuk University; Scientific Research Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipBlood samples of patients and healthy individuals for this study were kindly provided by University of Selcuk, Medical Faculty, Meram, Konya, Turkey. We would like to thank the Scientific Research Foundation of Selcuk University (BAP) for supporting this study. We also thank BioRad company for excellent technical assistance.en_US
dc.identifier.doi10.4238/2012.March.14.1en_US
dc.identifier.endpage590en_US
dc.identifier.issn1676-5680en_US
dc.identifier.issue1en_US
dc.identifier.pmid22535393en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage582en_US
dc.identifier.urihttps://dx.doi.org/10.4238/2012.March.14.1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28380
dc.identifier.volume11en_US
dc.identifier.wosWOS:000301923500062en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherFUNPEC-EDITORAen_US
dc.relation.ispartofGENETICS AND MOLECULAR RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDiabetes mellitusen_US
dc.subjectHypertensionen_US
dc.subjectVDR geneen_US
dc.subjectRT-qPCRen_US
dc.subjectPolymorphismen_US
dc.titleRT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkeyen_US
dc.typeArticleen_US

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