Synthesis and characterization of PS-b-PGMA diblock copolymer and its interaction with blood proteins and donepezil
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE BV
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Biocompatible polystyrene-block-poly(glycidyl methacrylate) (PS-b-PGMA) diblock copolymers with different molecular weights were synthesized via atom transfer radical polymerization (ATRP), homopolymers of styrene with narrow molecular weight distribution were prepared and used macroinitiators for block copolymerization. The effect of time, temperature, solvent, and in-feed ratio on the synthesis of the polymer was examined. Human serum albumin (HSA), bovine serum albumin (BSA), globulin (Gib) and hemoglobin (Hb) which are intrinsically fluorescent blood proteins were interacted with the synthesized PS-b-PGMA diblock copolymers. A calibration curve equation was used to calculate the amount of immobilized proteins on the diblock copolymers through fluorescence spectroscopy. These occurred at wavelengths of 280 nm and 324 nm, which correspond to the excitation and emission wavelengths of tyrosine and tryptophan residues. Following the interaction of PS-b-PGMA diblock copolymer with the blood proteins, the protein was subjected to a pharmaceutical active substance, donepezil. The copolymers, homo polymers and protein-polymers along with the drug interactions were characterized using H-1 NMR, FT-IR and gel permeation chromatography (GPC), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). (C) 2014 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
ATRP, Block copolymerization, Donepezil, Glycidyl methacrylate
Kaynak
REACTIVE & FUNCTIONAL POLYMERS
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
82
