Synthesis and characterization of PS-b-PGMA diblock copolymer and its interaction with blood proteins and donepezil

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dc.contributor.authorBuzoğlu, Leman
dc.contributor.authorMaltaş, Esra
dc.contributor.authorErsöz, Mustafa
dc.contributor.authorYıldız, Salih
dc.date.accessioned2020-03-26T18:58:33Z
dc.date.available2020-03-26T18:58:33Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBiocompatible polystyrene-block-poly(glycidyl methacrylate) (PS-b-PGMA) diblock copolymers with different molecular weights were synthesized via atom transfer radical polymerization (ATRP), homopolymers of styrene with narrow molecular weight distribution were prepared and used macroinitiators for block copolymerization. The effect of time, temperature, solvent, and in-feed ratio on the synthesis of the polymer was examined. Human serum albumin (HSA), bovine serum albumin (BSA), globulin (Gib) and hemoglobin (Hb) which are intrinsically fluorescent blood proteins were interacted with the synthesized PS-b-PGMA diblock copolymers. A calibration curve equation was used to calculate the amount of immobilized proteins on the diblock copolymers through fluorescence spectroscopy. These occurred at wavelengths of 280 nm and 324 nm, which correspond to the excitation and emission wavelengths of tyrosine and tryptophan residues. Following the interaction of PS-b-PGMA diblock copolymer with the blood proteins, the protein was subjected to a pharmaceutical active substance, donepezil. The copolymers, homo polymers and protein-polymers along with the drug interactions were characterized using H-1 NMR, FT-IR and gel permeation chromatography (GPC), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University, Konya, Turkey (BAP)Selcuk University [12101023]en_US
dc.description.sponsorshipWe thank the Scientific Research Foundation of Selcuk University, Konya, Turkey (BAP-Grant No.: 12101023) for financial support. This work is a part of the M.Sc. thesis of Leman Buzoglu. The authors also thank to Murat Kurnaz, Dr. Mustafa Ozmen and Dr. Salih Zeki Bas for editing this manuscript.en_US
dc.identifier.doi10.1016/j.reactfunctpolym.2014.05.009en_US
dc.identifier.endpage32en_US
dc.identifier.issn1381-5148en_US
dc.identifier.issn1873-166Xen_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.reactfunctpolym.2014.05.009
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31169
dc.identifier.volume82en_US
dc.identifier.wosWOS:000341895700004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofREACTIVE & FUNCTIONAL POLYMERSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectATRPen_US
dc.subjectBlock copolymerizationen_US
dc.subjectDonepezilen_US
dc.subjectGlycidyl methacrylateen_US
dc.titleSynthesis and characterization of PS-b-PGMA diblock copolymer and its interaction with blood proteins and donepezilen_US
dc.typeArticleen_US

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