Identification of factor XI deficiency in Holstein cattle in Turkey

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dc.contributor.authorMeydan, Hasan
dc.contributor.authorYildiz, Mehmet A.
dc.contributor.authorOzdil, Fulya
dc.contributor.authorGedik, Yasemin
dc.contributor.authorOzbeyaz, Ceyhan
dc.date.accessioned2020-03-26T17:38:53Z
dc.date.available2020-03-26T17:38:53Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Factor XI (FXI) is a plasma protein that participates in the formation of blood clots. Factor XI deficiency is autosomal recessive hereditary disorder that may be associated with excess bleeding in Holstein cattle. Methods: In this study, 225 Holstein cows reared in Turkey were screened in order to identify FXI genotypes. DNA extractions were obtained from the fresh blood of the cows. Amplicons of FXI exon 12 were obtained by Polymerase Chain Reaction (PCR), and analyzed by 2% agarose gel electrophoresis stained with ethidium bromide. Additionally, all cows were confirmed by DNA sequencing to determine whether or not there was a mutant allele. Results: Carriers of the FXI deficiency have two DNA fragments of 320 bp and 244 bp in size. The results of our study demonstrated that only four out of the 225 Holstein cows tested in Turkey carried the FXI deficiency. The frequency of the mutant FXI allele and the prevalence of heterozygous cows were found as 0.9% and 1.8%, respectively. Conclusion: The DNA-based test determines all genotypes, regardless of phenotype or FXI activity. The mutation responsible for the FXI deficiency had not been detected in Holstein cattle in Turkey before prior to this study. The frequency of the mutant FXI allele needs to be confirmed by carrying out further analyses on cattle in Turkey and the selection programs should be developed to eliminate this genetic disorder.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-107O914]en_US
dc.description.sponsorshipWe are indebted to Dr. Jonathan E. Beever of the Department of Animal Sciences, University of Illinois, Urbana-USA, for providing the control DNA samples. This study was partially supported by a grant, TOVAG-107O914, from The Scientific and Technological Research Council of Turkey (Project coordinator: Dr. M. A. YILDIZ). We are also grateful to Dr. Hans H. Cheng of the USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, Michigan State University, and anonymous reviewers for their comments on an earlier version of the manuscript.en_US
dc.identifier.doi10.1186/1751-0147-51-5en_US
dc.identifier.issn0044-605Xen_US
dc.identifier.pmid19161609en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1186/1751-0147-51-5
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23596
dc.identifier.volume51en_US
dc.identifier.wosWOS:000263951400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBIOMED CENTRAL LTDen_US
dc.relation.ispartofACTA VETERINARIA SCANDINAVICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleIdentification of factor XI deficiency in Holstein cattle in Turkeyen_US
dc.typeArticleen_US

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